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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8 Pt 1
|
| pubmed:dateCreated |
1997-9-11
|
| pubmed:abstractText |
The Hitachi L-8500A is a newly available apparatus for amino acid (AA) analysis that allows automatic on-line mixing of the ninhydrin reagent. The within-run precision (human plasma pools at three different concentrations) showed CVs < 3.8% except for the lowest concentration of citrulline (4.4%), Tyr (4.5%), and alpha-aminobutyric acid (7.6%), and for the intermediate concentration of Asp (8.7%). Between-run precision (CV) was < 3.1% for 17 AAs and < 8.0% for 24 of 25 AAs (CV Asp = 12.0%). For retention times, within-run precision was < 0.4% and between-run precision < 1.8%. Excellent relations were found between the results from the Hitachi L-8500A and the widely used Beckman 6300 analyzer (0.929 < or = r < or = 0.999). The detection was still linear at 5 mumol/L except for Pro and hydroxyproline (20 mumol/L). The upper limit was at least 2500 mumol/L for 13 AAs and at least 1000 mumol/L for 27 of 29 AAs (anserine = 500, Val = 600 mumol/L). Values from 100 human plasma samples agreed with previously published data. We conclude that the results obtained with the Hitachi L-8500A are satisfactory when compared with those of other AA analyzers utilizing the same method. Furthermore, the Hitachi L-8500A displays several advantages including programming flexibility, microsample capacity, low noise plotting, ammonia filtering, and manual repacking of the analytical column.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyproline,
http://linkedlifedata.com/resource/pubmed/chemical/Ninhydrin,
http://linkedlifedata.com/resource/pubmed/chemical/Proline
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0009-9147
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
43
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1421-8
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9267323-Amino Acids,
pubmed-meshheading:9267323-Automation,
pubmed-meshheading:9267323-Body Fluids,
pubmed-meshheading:9267323-Buffers,
pubmed-meshheading:9267323-Calibration,
pubmed-meshheading:9267323-Chromatography, High Pressure Liquid,
pubmed-meshheading:9267323-Chromatography, Ion Exchange,
pubmed-meshheading:9267323-Evaluation Studies as Topic,
pubmed-meshheading:9267323-Humans,
pubmed-meshheading:9267323-Hydroxyproline,
pubmed-meshheading:9267323-Ninhydrin,
pubmed-meshheading:9267323-Proline,
pubmed-meshheading:9267323-Regression Analysis,
pubmed-meshheading:9267323-Reproducibility of Results,
pubmed-meshheading:9267323-Sensitivity and Specificity
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Amino acid determination in biological fluids by automated ion-exchange chromatography: performance of Hitachi L-8500A.
|
| pubmed:affiliation |
INSERM U402, CHU Saint-Antoine, Paris, France.
|
| pubmed:publicationType |
Journal Article
|