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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-9-8
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pubmed:abstractText |
Gastric acid secretion is mediated by the H/K-ATPase of parietal cells. Activation of acid secretion involves insertion of H/K-ATPase into the parietal cell plasmalemma, while its cessation is associated with reinternalization of the H/K-ATPase into an intracellular storage compartment. The cytoplasmic tail of the H/K-ATPase beta subunit includes a four residue sequence homologous to tyrosine-based endocytosis signals. We generated transgenic mice expressing H/K-ATPase beta subunit in which this motif's tyrosine residue is mutated to alanine. Gastric glands from animals expressing mutant beta subunit constitutively secrete acid and continuously express H/K-ATPase at their cell surfaces. Thus, the beta subunit's tyrosine-based signal is required for the internalization of H/K-ATPase and for the termination of acid secretion. As a consequence of chronic hyperacidity, the mice develop gastric ulcers and a hypertrophic gastropathy resembling Menetrier's disease.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
501-10
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9267030-Animals,
pubmed-meshheading:9267030-COS Cells,
pubmed-meshheading:9267030-Calcium,
pubmed-meshheading:9267030-Cytomegalovirus,
pubmed-meshheading:9267030-DNA Primers,
pubmed-meshheading:9267030-Endocytosis,
pubmed-meshheading:9267030-Gastric Acid,
pubmed-meshheading:9267030-Gastric Mucosa,
pubmed-meshheading:9267030-H(+)-K(+)-Exchanging ATPase,
pubmed-meshheading:9267030-Macromolecular Substances,
pubmed-meshheading:9267030-Mice,
pubmed-meshheading:9267030-Mice, Transgenic,
pubmed-meshheading:9267030-Microscopy, Immunoelectron,
pubmed-meshheading:9267030-Mutagenesis, Site-Directed,
pubmed-meshheading:9267030-Parietal Cells, Gastric,
pubmed-meshheading:9267030-Polymerase Chain Reaction,
pubmed-meshheading:9267030-Promoter Regions, Genetic,
pubmed-meshheading:9267030-Recombinant Proteins,
pubmed-meshheading:9267030-Signal Transduction,
pubmed-meshheading:9267030-Transfection,
pubmed-meshheading:9267030-Tyrosine
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pubmed:year |
1997
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pubmed:articleTitle |
A tyrosine-based signal targets H/K-ATPase to a regulated compartment and is required for the cessation of gastric acid secretion.
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pubmed:affiliation |
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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