Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-9-18
|
| pubmed:abstractText |
Flow cytometric study revealed that almost all CD34+ cells in human umbilical cord blood expressed interferon-gamma receptor (IFN-gammaR). To clarify the precise functional roles of IFN-gammaR in human CD34+ cells, we examined the effect of IFN-gamma alone and in combination with various cytokines on the growth of haemopoietic progenitor cells in CD34+ cells using a serum-free clonal culture. Surprisingly, IFN-gamma alone supported only megakaryocyte (MK) colonies in a dose-dependent manner with a plateau level at 1000 U/ml of IFN-gamma. IFN-gamma at 1000 U/ml induced 10 +/- 1.2 MK colonies from 1 x 10(3) CD34+ cells, whereas thrombopoietin (TPO), interleukin (IL)-3, stem cell factor (SCF) or IL-6 alone induced 22 +/- 4.0, 22 +/- 4.2, 4 +/- 0.6 and 0 MK colonies, respectively. The addition of anti-IFN-gamma monoclonal antibody (mAb) to the IFN-gamma culture completely abrogated MK colony formation, whereas the mAb had no effect on TPO-dependent production of MK colonies. In contrast, although anti-TPO polyclonal Ab almost completely blocked TPO-dependent MK colony formation, it failed to inhibit the generation of MK colonies induced by IFN-gamma, suggesting that the observed effect of IFN-gamma on the proliferation of human MK progenitor cells is independent of TPO. The addition of IFN-gamma to culture with TPO or SCF significantly augmented the development of MK colonies, whereas it did not affect IL-3-dependent MK colony formation. Additionally, IFN-gamma induced the increase of DNA content of cultured glycoprotein IIb/IIIa-positive megakaryocytes. These results suggest that IFN-gamma may have regulatory roles in human megakaryocytopoiesis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Glycoprotein GPIIb-IIIa...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interferon,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombopoietin,
http://linkedlifedata.com/resource/pubmed/chemical/interferon gamma receptor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0007-1048
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
98
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
265-73
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9266918-Antibodies, Monoclonal,
pubmed-meshheading:9266918-Antigens, CD34,
pubmed-meshheading:9266918-Cell Division,
pubmed-meshheading:9266918-Culture Media, Serum-Free,
pubmed-meshheading:9266918-DNA,
pubmed-meshheading:9266918-Dose-Response Relationship, Drug,
pubmed-meshheading:9266918-Drug Synergism,
pubmed-meshheading:9266918-Flow Cytometry,
pubmed-meshheading:9266918-Hematopoietic Stem Cells,
pubmed-meshheading:9266918-Humans,
pubmed-meshheading:9266918-Interferon-gamma,
pubmed-meshheading:9266918-Interleukin-3,
pubmed-meshheading:9266918-Interleukin-6,
pubmed-meshheading:9266918-Megakaryocytes,
pubmed-meshheading:9266918-Mitosis,
pubmed-meshheading:9266918-Platelet Glycoprotein GPIIb-IIIa Complex,
pubmed-meshheading:9266918-Receptors, Interferon,
pubmed-meshheading:9266918-Stem Cell Factor,
pubmed-meshheading:9266918-Thrombopoietin
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Thrombopoietin-independent effect of interferon-gamma on the proliferation of human megakaryocyte progenitors.
|
| pubmed:affiliation |
Department of Clinical Oncology, Institute of Medical Science, University of Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|