Linked Life Data

9266797

Source:http://linkedlifedata.com/resource/pubmed/id/9266797

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-9-12
pubmed:abstractText
Chronic exposure to cadmium impairs various renal functions, including phosphate (Pi) transport. To further investigate the mechanism of cadmium-induced alterations in renal Pi transport, kinetics of Na+-dependent Pi uptake were studied in renal cortical brush-border membrane vesicles (BBMV) exposed to CdCl2 in vitro. BBMVs isolated from rabbit renal outer cortex were preincubated in a buffer containing CdCl2 (50 microM in most cases) for 60 min at 37 degrees C and then tested for Pi uptake at 25 degrees C. CdCl2 treatment resulted in a marked attenuation of Na+-dependent Pi uptake with no changes in Na+-independent Pi uptake and membrane permeability to Na+. CdMt treatment induced no changes in Pi transport. The inhibition required preincubation of vesicles with CdCl2 for more than 30 min and was not reversed by extravesicular EDTA, suggesting that cadmium affects the transport system at the internal side of the membrane. Kinetic analysis indicated that two sodium ions and one phosphate ion interact with a carrier, and this stoichiometry was not altered by cadmium treatment. Cadmium treatment did not change the apparent Km for Na+ (K(Na)) and that for phosphate (K(Pi)), but it markedly reduced the Vmax of the Na+-dependent Pi transport. These results indicate that exposure of proximal tubular brush border membranes to cadmium impairs the Pi transport capacity, probably by reducing the effective number of Na-Pi cotransporter units without altering substrate affinities of the carrier.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
255-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Effect of cadmium on Na-Pi cotransport kinetics in rabbit renal brush-border membrane vesicles.
pubmed:affiliation
Department of Physiology, Kosin Medical College, Pusan, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't