|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1997-10-15
|
|
pubmed:abstractText |
Four transpositional mutants of aniline-assimilating Pseudomonas sp. AW-2 produced 2-hydroxymuconic 6-semialdehyde (HMS) from aniline and accumulated it in a cultural medium. Among the four mutants, strain Y-2 produced the greatest amount of HMS (0.77 mg/ml) from 1 mg/ml of aniline hydrochloride in a 15-h growing culture. The conversion rate of aniline to HMS was 70% on a molar basis. Resting cells of strain Y-2 produced 0.65 mg/ml of HMS during 4h of incubation in a reaction mixture containing 1 mg/ml of aniline hydrochloride (conversion rate, 60%). Transposon Tn5-Mob was found to be inserted into the gene of HMS dehydrogenase in strain Y-2 by sequence analysis.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0944-5013
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
152
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
129-35
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:9265767-Alcohol Oxidoreductases,
pubmed-meshheading:9265767-Aldehyde Oxidoreductases,
pubmed-meshheading:9265767-Amino Acid Sequence,
pubmed-meshheading:9265767-Aniline Compounds,
pubmed-meshheading:9265767-Base Sequence,
pubmed-meshheading:9265767-Cloning, Molecular,
pubmed-meshheading:9265767-Fatty Acids, Unsaturated,
pubmed-meshheading:9265767-Molecular Sequence Data,
pubmed-meshheading:9265767-Mutagenesis, Insertional,
pubmed-meshheading:9265767-Mutation,
pubmed-meshheading:9265767-Pseudomonas,
pubmed-meshheading:9265767-Sequence Analysis, DNA
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
A high level of accumulation of 2-hydroxymuconic 6-semialdehyde from aniline by the transpositional mutant Y-2 of Pseudomonas species AW-2.
|
|
pubmed:affiliation |
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan.
|
|
pubmed:publicationType |
Journal Article
|
