Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1997-10-2
|
| pubmed:abstractText |
Nitric oxide (NO) synthase inhibitors, such as NG-nitro-L-arginine methyl ester (L-NAME), have been shown to attenuate endotoxin-induced uveitis (EIU) but they could increase leukocyte adhesion to the vascular endothelium. We hypothesize that a concomitant treatment with the 5-lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA) in 50% dimethylsulfoxide (DMSO, a hydroxyl radical scavenger) could improve the anti-inflammatory activity of L-NAME. EIU was induced in albino rabbits by intravitreal injection of 100 ng lipopolysaccharide. Animals were treated with multiple intraperitoneal injections of 50% DMSO in phosphate-buffered saline (PBS), NDGA (10 mg/kg) in 50% DMSO, L-NAME (50 mg/ kg) in PBS, or the combination NDGA+L-NAME. Uveitis was assessed by slit lamp examination, protein levels in aqueous humor, and myeloperoxidase (MPO) activity in the iris/ciliary body 6 h after induction. Nitrite, leukotriene B4 (LTB4), prostaglandin E2 (PGE2), platelet-activating factor (PAF) and interleukin-1 beta (IL-1 beta) levels in aqueous humor were also determined. NDGA or L-NAME alone did not show a significant reduction of uveitis intensity, although a significant decrease in MPO or in proteins was found, respectively. The combination NDGA+L-NAME significantly reduced the uveitis intensity, MPO in the iris/ciliary body, and the levels of nitrites, LTB4, PGE2, and PAF in aqueous humor. IL-1 beta levels were lower than the detection limit of the radioimmunoassay in all treatment groups. We conclude that concomitant treatment with NDGA in DMSO improves the anti-inflammatory activity of L-NAME during the early phase of EIU, suggesting that the inhibition of NO synthesis could enhance leukocyte infiltration and the release of oxygen free radicals.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Dimethyl Sulfoxide,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Endotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Leukotriene B4,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nordihydroguaiaretic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxidase
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0030-3747
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
29
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
227-36
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:9261846-Administration, Topical,
pubmed-meshheading:9261846-Animals,
pubmed-meshheading:9261846-Anti-Inflammatory Agents,
pubmed-meshheading:9261846-Aqueous Humor,
pubmed-meshheading:9261846-Ciliary Body,
pubmed-meshheading:9261846-Dimethyl Sulfoxide,
pubmed-meshheading:9261846-Dinoprostone,
pubmed-meshheading:9261846-Disease Models, Animal,
pubmed-meshheading:9261846-Drug Therapy, Combination,
pubmed-meshheading:9261846-Endothelium, Vascular,
pubmed-meshheading:9261846-Endotoxins,
pubmed-meshheading:9261846-Enzyme Inhibitors,
pubmed-meshheading:9261846-Interleukin-1,
pubmed-meshheading:9261846-Iris,
pubmed-meshheading:9261846-Leukotriene B4,
pubmed-meshheading:9261846-Lipoxygenase Inhibitors,
pubmed-meshheading:9261846-Male,
pubmed-meshheading:9261846-NG-Nitroarginine Methyl Ester,
pubmed-meshheading:9261846-Nitric Oxide Synthase,
pubmed-meshheading:9261846-Nordihydroguaiaretic Acid,
pubmed-meshheading:9261846-Peroxidase,
pubmed-meshheading:9261846-Rabbits,
pubmed-meshheading:9261846-Salmonella typhimurium,
pubmed-meshheading:9261846-Treatment Outcome,
pubmed-meshheading:9261846-Uveitis
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Concomitant treatment with a 5-lipoxygenase inhibitor improves the anti-inflammatory effect of the inhibition of nitric oxide synthase during the early phase of endotoxin-induced uveitis in the rabbit.
|
| pubmed:affiliation |
Ciba Vision, Barcelona, Spain.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|