|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0012591,
umls-concept:C0033414,
umls-concept:C0206558,
umls-concept:C0268140,
umls-concept:C0599773,
umls-concept:C0851827,
umls-concept:C1333231,
umls-concept:C1333358,
umls-concept:C1519249,
umls-concept:C1701901,
umls-concept:C1705469
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
1997-9-17
|
|
pubmed:abstractText |
We have examined mechanisms of recombination in mammalian cells infected with herpes simplex virus type 1 (HSV-1). Amplification of plasmids containing a viral origin of replication, oriS, in cells superinfected with HSV-1 revealed that linear DNA could be efficiently converted to templates for replication. Two distinct pathways were observed: imprecise end joining and nonconservative homologous recombination. We noted that direct repeats of the viral a sequence promoted efficient nonconservative homologous recombination in BHK cells as well as human repair-proficient 1BR.3N cells and xeroderma pigmentosum group F (XP-F) cells. The reaction gave rise to functional a sequences supporting the formation of defective viruses. It did not seem to proceed by single-strand annealing since it occurred in the absence of XPF/ERCC4, the mammalian homolog of the Rad1 endonuclease from Saccharomyces cerevisiae. In contrast, direct repeats of a 161-bp nonviral sequence did not take part in nonconservative homologous recombination in XP-F cells. Our results suggest that homologous recombination may be involved in the circularization of viral genomes. Furthermore, they demonstrate that amplification of recombination products supported by HSV-1 allows a direct examination of pathways for double-strand-break repair in human cells.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-1309247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-1323316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-1411547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-1850034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-2168985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-2840204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-2982037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-2987922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-2988788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-3016310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-3037530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-3561389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-4291934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-6096689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-6297756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-6330525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-6380756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-7482764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-7583098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-7707536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-7809076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-7891718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-7958917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8178432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8197110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8212585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8254746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8389882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8396795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8676478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8676928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8692798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8797827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8811177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8943369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8955060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8971009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261409-8978601
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
71
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
6842-9
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:9261409-Animals,
pubmed-meshheading:9261409-Cell Line,
pubmed-meshheading:9261409-Cricetinae,
pubmed-meshheading:9261409-DNA, Circular,
pubmed-meshheading:9261409-DNA, Viral,
pubmed-meshheading:9261409-DNA Replication,
pubmed-meshheading:9261409-DNA-Binding Proteins,
pubmed-meshheading:9261409-Defective Viruses,
pubmed-meshheading:9261409-Genes, Viral,
pubmed-meshheading:9261409-Herpesvirus 1, Human,
pubmed-meshheading:9261409-Humans,
pubmed-meshheading:9261409-Plasmids,
pubmed-meshheading:9261409-Recombination, Genetic,
pubmed-meshheading:9261409-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9261409-Replication Origin,
pubmed-meshheading:9261409-Virus Replication
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Direct repeats of the herpes simplex virus a sequence promote nonconservative homologous recombination that is not dependent on XPF/ERCC4.
|
|
pubmed:affiliation |
Department of Medical Biochemistry, University of Göteborg, Sweden.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
