Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1997-9-17
pubmed:abstractText
The herpes simplex virus type 1 (HSV-1) UL8 DNA replication protein is a component of a trimeric helicase-primase complex. Sixteen UL8-specific monoclonal antibodies (MAbs) were isolated and characterized. In initial immunoprecipitation experiments, one of these, MAb 804, was shown to coprecipitate POL, the catalytic subunit of the HSV-1 DNA polymerase, from extracts of insect cells infected with recombinant baculoviruses expressing the POL and UL8 proteins. Coprecipitation of POL was dependent on the presence of UL8 protein. Rapid enzyme-linked immunosorbent assays (ELISAs), in which one protein was bound to microtiter wells and binding of the other protein was detected with a UL8- or POL-specific MAb, were developed to investigate further the interaction between the two proteins. When tested in the ELISAs, five of the UL8-specific MAbs consistently inhibited the interaction, raising the possibility that these antibodies act by binding to epitopes at or near a site(s) on UL8 involved in its interaction with POL. The epitopes recognized by four of the inhibitory MAbs were approximately located by using a series of truncated UL8 proteins expressed in mammalian cells. Three of these MAbs recognized an epitope near the C terminus of UL8, which was subjected to fine mapping with a series of overlapping peptides. The C-terminal peptides were then tested in the ELISA for their ability to inhibit the POL-UL8 interaction: the most potent exhibited a 50% inhibitory concentration of approximately 5 microM. Our findings suggest that the UL8 protein may be involved in recruiting HSV-1 DNA polymerase into the viral DNA replication complex and also identify a potential new target for antiviral therapy.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-1172191, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-1309257, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-1311360, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-1312116, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-1321275, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-1657954, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-1707429, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-1847509, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-1867862, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-2163521, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-2170378, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-2173776, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-2216760, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-2538835, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-2546125, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-2556383, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-2826806, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-2987682, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-3012359, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-3012360, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-3553425, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-6323024, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-6329712, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-7658449, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-7775476, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-7878000, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-7931156, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-7961904, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-7964622, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-7990963, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8106478, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8189507, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8380075, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8380085, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8380091, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8382792, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8385691, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8396795, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8397405, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8599237, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8627697, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8627698, http://linkedlifedata.com/resource/pubmed/commentcorrection/9261356-8811024
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6390-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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