Linked Life Data

9261134

Source:http://linkedlifedata.com/resource/pubmed/id/9261134

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
34
pubmed:dateCreated
1997-9-15
pubmed:abstractText
Studies under blockade of alpha-, beta1-, and beta2-adrenoreceptors revealed a good correlation between the responses of rat colon relaxation of depolarized tonus and of rat adipocyte lipolysis elicited by catecholamines or BRL-37344, a selective beta3-adrenoreceptor agonist, suggesting beta3-adrenoreceptor stimulation. In contrast, SM-11044, a nonselective beta-adrenoreceptor agonist, stimulated colon relaxation more efficiently than lipolysis; its effects were differently antagonized by cyanopindolol with pA2 values of 8.31 in colon and of 7.32 in adipocytes. Binding studies in rat colon smooth muscle membranes using [125I]iodocyanopindolol under blockade of adrenaline and serotonin receptors revealed the existence of a single class of sites (Kd = 11.0 nM, Bmax = 716.7 fmol/mg protein). The specific binding was saturable and reversible and was displaced by SM-11044 but not by BRL-37344, isoproterenol, noradrenaline, adrenaline, serotonin, nor dopamine. This binding site was photoaffinity labeled using [125I]iodocyanopindolol-diazirine. The labeling was prevented by SM-11044 but not by BRL-37344. The amino-terminal amino acid sequences of the high performance liquid chromatography-purified peptides generated by enzymatic and chemical cleavages of the affinity labeled 34-kDa protein confirmed that the novel iodocyanopindolol or SM-11044 binding protein of rat colon smooth muscle membranes is different from known adrenaline, serotonin, or dopamine receptors. Its functional role might include the relaxation of depolarized colon.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21244-52
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9261134-Adipocytes, pubmed-meshheading:9261134-Adrenergic beta-Agonists, pubmed-meshheading:9261134-Affinity Labels, pubmed-meshheading:9261134-Amino Acid Sequence, pubmed-meshheading:9261134-Animals, pubmed-meshheading:9261134-Carrier Proteins, pubmed-meshheading:9261134-Catechols, pubmed-meshheading:9261134-Cell Membrane, pubmed-meshheading:9261134-Cell-Free System, pubmed-meshheading:9261134-Colon, pubmed-meshheading:9261134-Iodocyanopindolol, pubmed-meshheading:9261134-Molecular Sequence Data, pubmed-meshheading:9261134-Molecular Weight, pubmed-meshheading:9261134-Muscle, Smooth, pubmed-meshheading:9261134-Muscle Tonus, pubmed-meshheading:9261134-Peptide Mapping, pubmed-meshheading:9261134-Phentolamine, pubmed-meshheading:9261134-Pindolol, pubmed-meshheading:9261134-Propranolol, pubmed-meshheading:9261134-Protein Binding, pubmed-meshheading:9261134-Rats, pubmed-meshheading:9261134-Serine, pubmed-meshheading:9261134-Serotonin, pubmed-meshheading:9261134-Solubility
pubmed:year
1997
pubmed:articleTitle
Characterization of a novel iodocyanopindolol and SM-11044 binding protein, which may mediate relaxation of depolarized rat colon tonus.
pubmed:affiliation
Institut Cochin de Génétique Moléculaire, CNRS-UPR 0415 and Université Paris VII, 22 rue Méchain, 75014 Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't