Linked Life Data

9260739

Source:http://linkedlifedata.com/resource/pubmed/id/9260739

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-9-26
pubmed:abstractText
The neurotrophin receptor TrkB plays a key role in promoting cell survival and differentiation in the nervous system. Two adhesive motifs in the extracellular domain of TrkB have been proposed based on its predicted secondary structure. To investigate the potential adhesive function of trkB, a full length trkB cDNA was stably transfected into NIH 3T3 cells and TrkB-expressing clones isolated. Transfectant clones producing different levels of TrkB protein were subjected to a homotypic aggregation assay. Results showed that parental cells were non-adhesive during the assay while TrkB-expressing cells displayed varying degrees of aggregation depending on the amount of TrkB protein expressed. The observed adhesion was Ca(2+)-, Mg(2+)-, and temperature-dependent, characteristics shared by the cadherin family of adhesion molecules. The transfected cell lines also expressed cadherin in proportion to TrkB expression and both molecules were required for cell adhesion. Double immunofluorescence staining studies showed that TrkB was colocalized with cadherin and catenin at cell-cell contact sites. Whether TrkB and cadherin mediate adhesion separately or synergistically remains to be determined.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-91
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
BDNF/NT4-5 receptor TrkB and cadherin participate in cell-cell adhesion.
pubmed:affiliation
Department of Neurobiology, Stanford University School of Medicine, California 94305-5401, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't