Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1980-2-28
pubmed:abstractText
The PLC/PRF/5 cell line derived from a human hepatoma produces hepatitis B surface antigen (HBsAg) in 22-nm particles of the same buoyant density as those found in the serum of infected patients. The HBsAg particles from this cell line were labeled with [35S]methionine and purified, and the polypeptides were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with those of serum-derived particles. The two major polypeptides of serum-derived HBsAg particles (p20 and p23) were found in the same relative amounts in the particles from the cell line. The three smallest of the five minor components observed in HBsAg particles from serum were present in particles from the cell line. These polypeptides (p31, p36, and p43), as well as p20 and p23, were precipitated with anti-HBs-containing serum. The two largest polypeptides of serum particles (p49 and p66) were not detected in particles from these cells. When the PLC/PRF/5 HBsAg particles were radiolabeled with tritiated sugars, p23, and not p20, was found to contain radioactivity, indicating that the pattern of polypeptide glycosylation is similar to that of serum HBsAg. None of the other possible gene products of hepatitis B virus was detected in the PLC/PRF/5-derived HBsAg particles, in the cells, or in the cell supernatants.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-105041, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-1089835, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-1102604, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-1151071, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-174434, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-187208, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-204663, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-212785, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-266193, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-334041, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-4512480, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-4574998, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-45864, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-4850204, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-4852257, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-4854012, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-51098, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-62001, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-850309, http://linkedlifedata.com/resource/pubmed/commentcorrection/92575-993789
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
796-802
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Polypeptides of hepatitis B virus surface antigen produced by a hepatoma cell line.
pubmed:publicationType
Journal Article