Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1978-1-27
pubmed:abstractText
Human urinary kallikrein (EC 3.4.21.8) was purified to electrophoretically homogeneity by gel filtration on Sephadex G-100, followed by affinity chromatography on aprotinin-Sepharose. The molecular weight, estimated by gel filtration, is 64,000 Daltons, by dodecyl sulfate electrophoresis 45,000 Daltons and 29,000 Daltons, and it is microheterogeneous on electrofocusing, yielding isoelectric point values of 3.8, 3.9 and 4.05. The kinetic constants for hydrolysis of BZArgOEt, TosArgOMe, CbzTyrONp, and human HMW-kininogen were determined. Benzamidine and aprotinin are competitive inhibitors of human urinary kallikrein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0340-076X
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
479-83
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Isolation of human urinary kallikrein by affinity chromatography. Determination of human urinary kallikrein, I.
pubmed:publicationType
Journal Article