Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1997-10-10
|
| pubmed:abstractText |
A novel saccharide was synthesized by incubating globo-N-tetraose, GalNAc beta1-3Gal alpha1-4Gal beta1-4Glc, and UDP[3H]GlcNAc with hog gastric mucosal microsomes, known to contain beta1,6-N-acetylglucosaminyltransferase activity of a broad acceptor specificity. Chromatography and MALDI-TOF mass spectrometry of the product, as well as the amount of incorporated radioactivity indicated that one [3H]GlcNAc residue was transferred to the acceptor saccharide. One- and two-dimensional 1H NMR-spectroscopic analysis of the product and ESI-CID mass spectrometry of the pentasaccharide in permethylated form established its structure as GalNAc beta1-3([3H]GlcNAc beta1-6)Gal alpha1-4Gal beta1-4Glc. The new enzyme activity possesses substrate specificity features common to a purified beta1,6-GlcNAc-transferase from bovine tracheal epithelium, which forms branches at the subterminal beta1,3-substituted galactose and accepts both GlcNAc- and Gal-configuration at the terminal residue of the acceptor (Ropp et al. (1991) J. Biol. Chem., 266, 23863-23871). The new beta1,6-GlcNAc-branch was readily galactosylated by bovine milk beta1,4-galactosyltransferase, revealing a pathway to novel hybrid type glycans with N-acetyllactosamine chains on globotype saccharides. This pathway may lead to the rare IP blood-group antigen and to globoside-like molecules mediating cell adhesion.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Globosides,
http://linkedlifedata.com/resource/pubmed/chemical/N-Acetylglucosaminyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium,
http://linkedlifedata.com/resource/pubmed/chemical/UDP-GlcNAc-Gb(5)Cer...,
http://linkedlifedata.com/resource/pubmed/chemical/Uridine Diphosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/globotetraose
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0959-6658
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
711-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9254053-Animals,
pubmed-meshheading:9254053-Carbohydrate Conformation,
pubmed-meshheading:9254053-Carbohydrate Sequence,
pubmed-meshheading:9254053-Cattle,
pubmed-meshheading:9254053-Epithelium,
pubmed-meshheading:9254053-Gastric Mucosa,
pubmed-meshheading:9254053-Globosides,
pubmed-meshheading:9254053-Magnetic Resonance Spectroscopy,
pubmed-meshheading:9254053-Microsomes,
pubmed-meshheading:9254053-Milk,
pubmed-meshheading:9254053-Molecular Sequence Data,
pubmed-meshheading:9254053-N-Acetylglucosaminyltransferases,
pubmed-meshheading:9254053-Oligosaccharides,
pubmed-meshheading:9254053-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:9254053-Substrate Specificity,
pubmed-meshheading:9254053-Swine,
pubmed-meshheading:9254053-Trachea,
pubmed-meshheading:9254053-Tritium,
pubmed-meshheading:9254053-Uridine Diphosphate N-Acetylglucosamine
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Enzymatic transfer of a beta1,6-linked N-acetylglucosamine to the alpha-galactose of globo-N-tetraose: in vitro synthesis of a novel hybrid pentasaccharide of lacto-globo type.
|
| pubmed:affiliation |
Institute of Biotechnology and Department of Biosciences (Division of Biochemistry), University of Helsinki, Finland.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|