Linked Life Data

9252961

Source:http://linkedlifedata.com/resource/pubmed/id/9252961

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1997-10-1
pubmed:abstractText
Several years ago, it was established that the delta 15 trans isomer of alpha-linolenic acid is converted in vivo into fatty acids containing 20 and 22 carbons (geometrical isomers of eicosapentaenoic and docosahexaenoic acids). The present study focused on the in vitro delta 6 desaturation, the first step of the biosynthesis of the n-3 long-chain polyunsaturated fatty acids from 18:3n-3. For that purpose, rat liver microsomes were prepared and incubated with radiolabeled 18:3 delta 9cis,12cis,15cis (18:3c,c,c) or 18:3 delta 9cis, 12cis, 15trans (18:3c,c,t) under desaturation conditions. The data show that 18:3c,c,t is converted at a lower rate compared with alpha-linolenic acid. The product of conversion of 18:3c,c,t may be 18:4 delta 6cis, 9cis, 12cis, 15trans resulting from a delta 6 desaturation of the trans substrate. Moreover, the conversion of radiolabeled 18:3c,c,t was strongly decreased by the presence of 18:3c,c,c (up to 48%) while the 18:3c,c,t only slightly decreased the conversion of radiolabeled 18:3c,c,c. Thus, the desaturation enzyme presented a higher affinity for the native all-cis n-3 substrate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0024-4201
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
731-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Conversion of 18:3 delta 9cis, 12cis, 15trans in rat liver microsomes.
pubmed:affiliation
INRA, Unité de Nutrition Lipidique, Dijon, France. chardign@dijon-inra.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't