Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-9-29
pubmed:abstractText
A general algebraic approach to the kinetic analysis of time-dependent absorption data is presented that allows the calculation of possible kinetic schemes. The kinetic matrices of all possible reaction mechanisms are calculated from experimental eigenvalues and eigenvectors derived from the decay constants and amplitude spectra (b-spectra) of the global exponential fit to the time-dependence of the absorption data. The eigenvalues are directly related to the decay constants, and the eigenvectors are obtained by decomposing the b-spectra into spectral components representing the intermediates. The analysis method is applied to the late intermediates (lumi, meta I, meta I-380, and meta II) of the rhodopsin photoreaction. The b-spectra are decomposed into lumi, meta I, meta-380, and rhodopsin spectra. The meta-380 component is partitioned into isospectral meta I-380 and meta II components based on physical criteria. The calculated kinetic matrices yield a number of reaction mechanisms (linear scheme with back reactions, branched schemes with equilibrium steps, and a variety of square models) consistent with the photolysis data at 25 degrees C. The problems associated with isospectral intermediates (meta I-380 and meta II) are treated successfully with this method.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-1337214, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-14080814, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-1565656, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-1645187, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-2009362, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-2271583, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-2334708, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-5913130, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-6572974, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-7787034, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-8268161, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-8547264, http://linkedlifedata.com/resource/pubmed/commentcorrection/9251787-9017194
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
688-702
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Deriving reaction mechanisms from kinetic spectroscopy. Application to late rhodopsin intermediates.
pubmed:affiliation
Department of Chemistry and Biochemistry, University of California, Santa Cruz 95064, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.