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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-10-23
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pubmed:abstractText |
In this study, we purified C-terminal propeptide of type I collagen (PICP) from the conditioned medium of osteoblastic MC3T3-E1 cells by chromatographic and Agarose gel extraction procedures. PICP was confirmed to be present in bone by Western blotting using a specific antibody, and was proved to be synthesized by osteoblasts with metabolic labeling. PICP promoted cell-attachment of osteoblastic MC3T3-E1 cells. We conclude that PICP is synthesized by osteoblasts and stored in bone, and that it plays a role in the maintenance of bone cells on bone matrix.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
1310
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
97-102
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:9244181-Amino Acid Sequence,
pubmed-meshheading:9244181-Animals,
pubmed-meshheading:9244181-Blotting, Western,
pubmed-meshheading:9244181-Bone Morphogenetic Proteins,
pubmed-meshheading:9244181-Bone and Bones,
pubmed-meshheading:9244181-Cell Adhesion,
pubmed-meshheading:9244181-Cell Line,
pubmed-meshheading:9244181-Culture Media,
pubmed-meshheading:9244181-Molecular Sequence Data,
pubmed-meshheading:9244181-Osteoblasts,
pubmed-meshheading:9244181-Peptide Fragments,
pubmed-meshheading:9244181-Procollagen,
pubmed-meshheading:9244181-Rats,
pubmed-meshheading:9244181-Tritium
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pubmed:year |
1996
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pubmed:articleTitle |
The osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen, which promoted cell-attachment of osteoblasts.
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pubmed:affiliation |
Department of Biochemistry, School of Dentistry, Hokkaido University, Saporro, Japan.
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pubmed:publicationType |
Journal Article
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