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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-9-3
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pubmed:abstractText |
L-type Ca2+ channel currents in cultured rat mesenteric artery smooth muscle cells were recorded by the cell-attached patch-clamp technique. Depolarizing voltage steps from a holding potential of -40 mV elicited voltage-dependent inward Ba2+ currents. The inward currents were inhibited by nifedipine (10 microM) but enhanced by Bay K 8644 (5 microM), which suggests that the inward currents are carried almost exclusively by L-type Ca2+ channels. Application of dibutyryl cAMP (0.1-1 microM) and forskolin (0.01-1 microM) enhanced the activity of these Ca2+ channels. The dibutyryl cAMP induced enhancement of Ca2+ channels was antagonized by the serine/threonine kinase inhibitor H-8 (1 microM). Application of 8-bromo-cGMP (0.01-1 microM) and the cGMP inducer nitroglycerin (0.01-1 microM) inhibited the activity of these Ca2+ channels, and the inhibition of channel activity induced by 8-bromo-cGMP was antagonized by the serine/threonine kinase inhibitor H-8 (1 microM). These results suggest that in rat mesenteric artery cells, the L-type Ca2+ channel current is enhanced by a rise in intracellular cAMP levels and suppressed by a rise in intracellular cGMP levels. Furthermore, cGMP-induced Ca2+ channel inhibition may play a role in the expression of the nitric oxide-mediated vasodilating action of drugs such as nitroglycerin and atrial natriuretic peptide.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-Pyridinecarboxylic acid...,
http://linkedlifedata.com/resource/pubmed/chemical/8-bromocyclic GMP,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic GMP,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/N-(2-(methylamino)ethyl)-5-isoquinol...,
http://linkedlifedata.com/resource/pubmed/chemical/Nifedipine,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroglycerin
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-5198
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
179-86
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9243326-3-Pyridinecarboxylic acid...,
pubmed-meshheading:9243326-Animals,
pubmed-meshheading:9243326-Bucladesine,
pubmed-meshheading:9243326-Calcium Channel Agonists,
pubmed-meshheading:9243326-Calcium Channel Blockers,
pubmed-meshheading:9243326-Calcium Channels,
pubmed-meshheading:9243326-Cyclic AMP,
pubmed-meshheading:9243326-Cyclic GMP,
pubmed-meshheading:9243326-Forskolin,
pubmed-meshheading:9243326-Isoquinolines,
pubmed-meshheading:9243326-Male,
pubmed-meshheading:9243326-Membrane Potentials,
pubmed-meshheading:9243326-Mesenteric Arteries,
pubmed-meshheading:9243326-Muscle, Smooth, Vascular,
pubmed-meshheading:9243326-Nifedipine,
pubmed-meshheading:9243326-Nitroglycerin,
pubmed-meshheading:9243326-Patch-Clamp Techniques,
pubmed-meshheading:9243326-Rats,
pubmed-meshheading:9243326-Rats, Wistar
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pubmed:year |
1997
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pubmed:articleTitle |
Effects of cAMP and cGMP on L-type calcium channel currents in rat mesenteric artery cells.
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pubmed:affiliation |
Department of Pharmacology, Showa College of Pharmaceutical Sciences, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro
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