Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-8-25
|
| pubmed:abstractText |
Human herpesvirus 6 (HHV-6) DNA has been detected in several human lymphoproliferative disorders. We report a case of HHV-6-infected Burkitt's lymphoma, from which a cell line, designated Katata, has been established. Katata cells had an immature B-cell phenotype with an L3 morphology and carried a t(8;14)(q24;q32) chromosomal abnormality. The HHV-6 DNA sequences were detected in both the patient's tumor cells and Katata cell line by polymerase chain reaction using three sets of primers that target different regions of HHV-6 DNA. The presence of HHV-6 DNA in Katata cells was also shown by Southern blot hybridization with the BamHI fragment of HHV-6. It is likely that the virus is in a latent state, since (1) virion-associated protein was not expressed in Katata cells, (2) transcriptional level of the immediate-early gene was very low, and (3) no viral particles were observed by electron microscopy. Katata cells were highly tumorigenic in nude mice and the tumor cells also contained HHV-6 DNA. We have successfully obtained several clonal lines by allowing the cells to form colonies in soft agarose and by the limiting dilution method. HHV-6 DNA was detectable in all 13 clones analyzed, suggesting that virtually all Katata cells are infected with HHV-6. This is the first report of a case of HHV-6+ Burkitt's lymphoma in the absence of Epstein-Barr virus. Furthermore, there has been no report of lymphoma cell lines that are persistently and nonproductively infected with HHV-6. The Katata Burkitt's lymphoma cell line, therefore, would provide a useful tool for studies of the mechanisms of HHV-6 latency and reactivation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
90
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1200-7
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9242553-Animals,
pubmed-meshheading:9242553-B-Lymphocytes,
pubmed-meshheading:9242553-Bone Marrow,
pubmed-meshheading:9242553-Burkitt Lymphoma,
pubmed-meshheading:9242553-Cell Differentiation,
pubmed-meshheading:9242553-DNA, Neoplasm,
pubmed-meshheading:9242553-DNA, Viral,
pubmed-meshheading:9242553-Fatal Outcome,
pubmed-meshheading:9242553-Female,
pubmed-meshheading:9242553-Herpesviridae Infections,
pubmed-meshheading:9242553-Herpesvirus 6, Human,
pubmed-meshheading:9242553-Humans,
pubmed-meshheading:9242553-Mice,
pubmed-meshheading:9242553-Mice, Nude,
pubmed-meshheading:9242553-Middle Aged,
pubmed-meshheading:9242553-Neoplasm Transplantation,
pubmed-meshheading:9242553-Neoplastic Stem Cells,
pubmed-meshheading:9242553-Phenotype,
pubmed-meshheading:9242553-Polymerase Chain Reaction,
pubmed-meshheading:9242553-Tumor Cells, Cultured,
pubmed-meshheading:9242553-Virus Latency
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Human herpesvirus 6 (HHV-6)-positive Burkitt's lymphoma: establishment of a novel cell line infected with HHV-6.
|
| pubmed:affiliation |
Department of Medicine, Kochi Medical School, Japan.
|
| pubmed:publicationType |
Journal Article,
Case Reports,
Research Support, Non-U.S. Gov't
|