Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-8-28
pubmed:databankReference
pubmed:abstractText
The differentiation of human embryo chondrocytes was markedly induced by the addition of Bt2cAMP to the culture medium. Using this culture system, a novel human cDNA for a basic helix-loop-helix (bHLH) protein (named DEC1) expressed primarily in the chondrocytes in response to Bt2cAMP was cloned by the subtractive hybridization method. DEC1 protein consists of 412 amino acids and exhibits structural similarities to the mammalian HES family, Drosophila hairy, and Enhancer of split m7 in the bHLH region. Northern blot analysis showed that DEC1 mRNA was expressed in various tissues including the cartilage, lung, spleen, and intestine, but not in the brain. These findings suggest that the bHLH factor DEC is involved in the control of cell differentiation in several tissues including cartilage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
236
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
294-8
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed-meshheading:9240428-Amino Acid Sequence, pubmed-meshheading:9240428-Base Sequence, pubmed-meshheading:9240428-Basic Helix-Loop-Helix Transcription Factors, pubmed-meshheading:9240428-Bucladesine, pubmed-meshheading:9240428-Cartilage, pubmed-meshheading:9240428-Cell Differentiation, pubmed-meshheading:9240428-Cells, Cultured, pubmed-meshheading:9240428-Cloning, Molecular, pubmed-meshheading:9240428-Epiphyses, pubmed-meshheading:9240428-Gene Expression Regulation, Developmental, pubmed-meshheading:9240428-Helix-Loop-Helix Motifs, pubmed-meshheading:9240428-Homeodomain Proteins, pubmed-meshheading:9240428-Humans, pubmed-meshheading:9240428-Molecular Sequence Data, pubmed-meshheading:9240428-Sequence Alignment, pubmed-meshheading:9240428-Sequence Homology, Amino Acid, pubmed-meshheading:9240428-Transcription Factors
pubmed:year
1997
pubmed:articleTitle
Molecular characterization of the novel basic helix-loop-helix protein DEC1 expressed in differentiated human embryo chondrocytes.
pubmed:affiliation
Department of Biochemistry, Hiroshima University School of Dentistry, Japan.
pubmed:publicationType
Journal Article