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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1997-8-21
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pubmed:abstractText |
Cell-Dyn 3500 automate (Abbott) provides reticulocyte count using a recent software, after ribosomal RNA colouring with methylene blue reagent. The aim of this study was to evaluate results from Cell-Dyn 3500, and to compare them with the routine laboratory technique by flow cytometry (FacScan, Becton-Dickinson). Cell-Dyn 3500 analytical parameters (inter- and intra-assay precision, sample-to-sample contamination) were good and in accord to manufacturer's specifications. Reticulocyte values from blood samples kept at 4 degrees C during 3 days were unchanged. Usual values in 145 normal patients were found between 71.3 and 78.3 G/l with Cell-Dyn 3500, between 119.3 and 130.2 G/l with FacScan. Both techniques were compared in 87 patients with various anemias: Cell-Dyn 3500 showed more frequently high reticulocyte values in regenerative anemias (hemorrhagic), but was less performant for classification of aregenerative anemias. Reticulocyte count with Cell-Dyn 3500 is a semi automatic, easy to perform and swift technique, using no toxic reagent but unitary test tubes kept at room temperature. The test cost is however high, and Cell-Dyn 3500 cannot be used during the analysis.
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pubmed:language |
fre
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
0003-3898
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
215-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading | |
pubmed:articleTitle |
[Evaluation of reticulocyte count on Cell-Dyn 3500 automate: comparison with a flow cytometry technique].
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pubmed:affiliation |
Laboratoire de biologie, Centre hospitalier Gilles-de-Corbeil, Corbeil-Essonnes.
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pubmed:publicationType |
Journal Article,
Comparative Study,
English Abstract
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