|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
16
|
|
pubmed:dateCreated |
1997-9-8
|
|
pubmed:abstractText |
The human androgen receptor (AR) is a ligand-activated transcription factor that regulates genes important for male sexual differentiation and development. To better understand the role of the receptor as a transcription factor we have studied the mechanism of action of the N-terminal transactivation function. In a protein-protein interaction assay the AR N terminus (amino acids 142-485) selectively bound to the basal transcription factors TFIIF and the TATA-box-binding protein (TBP). Reconstitution of the transactivation activity in vitro revealed that AR142-485 fused to the LexA protein DNA-binding domain was competent to activate a reporter gene in the presence of a competing DNA template lacking LexA binding sites. Furthermore, consistent with direct interaction with basal transcription factors, addition of recombinant TFIIF relieved squelching of basal transcription by AR142-485. Taken together these results suggest that one mechanism of transcriptional activation by the AR involves binding to TFIIF and recruitment of the transcriptional machinery.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-1775129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-1776169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-1985913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-2142753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-2163758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-2163759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-2510298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-2626022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-4075392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-7523389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-7553866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-7574492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-7589983,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-7671849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-7706276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-7854423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-7947816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-7971517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8114755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8133894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8146426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8165128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8187178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8205623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8252034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8352588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8417339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8609996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8612590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8628270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8628277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8630238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8643607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8668144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8732995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8755740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8783941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8794892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8870495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8870497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8870498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8900157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8943763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-8946909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-9010316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9238003-9046245
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
5
|
|
pubmed:volume |
94
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
8485-90
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:9238003-DNA-Binding Proteins,
pubmed-meshheading:9238003-Escherichia coli,
pubmed-meshheading:9238003-Humans,
pubmed-meshheading:9238003-Male,
pubmed-meshheading:9238003-Receptors, Androgen,
pubmed-meshheading:9238003-Recombinant Proteins,
pubmed-meshheading:9238003-Transcription Factors,
pubmed-meshheading:9238003-Transcription Factors, TFII,
pubmed-meshheading:9238003-Transcriptional Activation
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Interaction of the human androgen receptor transactivation function with the general transcription factor TFIIF.
|
|
pubmed:affiliation |
Department of Biosciences, Novum, Karolinska Institute, S-141 57 Huddinge, Sweden. iain.mcewan@abdn.ac.uk
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
