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rdf:type |
|
|
lifeskim:mentions |
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|
pubmed:issue |
2-3
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|
pubmed:dateCreated |
1997-9-3
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|
pubmed:abstractText |
In this report, cell-cycle-dependent effects of TFIID on other basal transcription factors were investigated. We purified TFIID fractions from HeLa cells synchronized in the S/G2 phases and in early G1 phase, and show that RAP74 is phosphorylated more highly by the S/G2 phase TFIID fraction than by the early G1 phase TFIID fraction. Further analyses using deletion mutants of RAP74 revealed that amino acid residues 206-256 are phosphorylated by the TFIID fraction. Reconstitution of in vitro transcription activity indicates that the cell-cycle-dependent phosphorylation of RAP74 increases TFIIF transcription activity.
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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|
pubmed:month |
Jun
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|
pubmed:issn |
0014-5793
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|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
30
|
|
pubmed:volume |
410
|
|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
477-80
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9237686-Animals,
pubmed-meshheading:9237686-Cell Cycle,
pubmed-meshheading:9237686-G2 Phase,
pubmed-meshheading:9237686-HeLa Cells,
pubmed-meshheading:9237686-Humans,
pubmed-meshheading:9237686-Phosphorylation,
pubmed-meshheading:9237686-S Phase,
pubmed-meshheading:9237686-Sequence Deletion,
pubmed-meshheading:9237686-Transcription Factor TFIID,
pubmed-meshheading:9237686-Transcription Factors,
pubmed-meshheading:9237686-Transcription Factors, TFII
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pubmed:year |
1997
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|
pubmed:articleTitle |
Cell-cycle-dependent phosphorylation of the basal transcription factor RAP74.
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|
pubmed:affiliation |
Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Japan. yonaha@dunn1.path.ox.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
