Linked Life Data

9237171

Source:http://linkedlifedata.com/resource/pubmed/id/9237171

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-9-4
pubmed:abstractText
An early step in a pathway leading to transcriptional initiation involves the rearrangement of chromatin at gene regulatory sequences. To study this process, we have developed a biochemical system analyzing the interactions between chromatin templates composed of arrays of positioned nucleosomes and sequence-specific transcriptional activators. Here, a procedure is presented for the assembly of nucleosomal arrays on DNA fragments containing synthetic and natural gene sequences inserted within tandem repeats of sea urchin 5S rDNA. We also provide methods for the use of these templates in transcription factor-binding assays, as well as experimental data illustrating the efficacy of such analyses to uncover mechanisms directing factor-mediated nucleosome remodeling.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1046-2023
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
276-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Analysis of transcription factor-mediated remodeling of nucleosomal arrays in a purified system.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802-4500, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't