Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-8-14
pubmed:abstractText
A Dynal core method has been established using Dynabeads anti-Salmonella to detect Salmonella from all categories of food samples. The protocol consists of the standard pre-enrichment of samples in buffered peptone water followed by immunomagnetic separation and subsequent selective enrichment of the bead-bacteria complexes in Rappaport-Vassiliadis Soya Peptone broth before plating onto Salmonella selective media. This modified IMS cultural method is intended to replace or augment traditional cultural methods used for Salmonella detection due to its specificity and increased sensitivity. The optional direct plating of bead-bacteria complexes onto solid media using a swab-streak technique is suitable for processed foods or samples known to have a history of very low resident flora. In an evaluation using 100 naturally contaminated samples, this IMS core method detected 39 of the 44 positive samples detected by all the methods combined, compared to 31 detected by the conventional ISO 6579 reference method. Furthermore, in ten different food matrices inoculated with low levels (1-5 cells/25 g) of twenty Salmonella serovariants, frozen for one month before being examined, the IMS core method, showed a 90% concordance with the ISO method and isolated two more Salmonella positive samples than the conventional ISO method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0168-1605
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-62
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Detection of Salmonella from raw food samples using Dynabeads anti-Salmonella and a conventional reference method.
pubmed:affiliation
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, Oslo, Norway. sewornu.cudjoe@veths.no
pubmed:publicationType
Journal Article