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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
7
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pubmed:dateCreated |
1997-8-14
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pubmed:abstractText |
A total of 173 Vibrio cholerae O1 isolates from the recent cholera epidemic in Colombia was analysed by the polymerase chain reaction (PCR) for the genes encoding the A subunit of cholera toxin (ctxA) and the zonula occludens toxin (zot), and by ribotyping. All isolates were positive for ctx A and zot, which was confirmed by hybridisation. Ribotyping with restriction endonuclease Bg/I digestion of total DNA revealed three ribotypes: B5a comprising 165 (96.4%) isolates, and two new designated ribotypes B20 and B21a in six (3.5%) isolates and two (1.1%) isolates, respectively. These findings have significant public health implications.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-2615
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
611-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9236746-Bacterial Typing Techniques,
pubmed-meshheading:9236746-Cholera,
pubmed-meshheading:9236746-Cholera Toxin,
pubmed-meshheading:9236746-Colombia,
pubmed-meshheading:9236746-Disease Outbreaks,
pubmed-meshheading:9236746-Genes, Bacterial,
pubmed-meshheading:9236746-Humans,
pubmed-meshheading:9236746-Molecular Epidemiology,
pubmed-meshheading:9236746-Polymerase Chain Reaction,
pubmed-meshheading:9236746-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:9236746-RNA, Bacterial,
pubmed-meshheading:9236746-RNA, Ribosomal,
pubmed-meshheading:9236746-Vibrio cholerae,
pubmed-meshheading:9236746-rRNA Operon
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pubmed:year |
1997
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pubmed:articleTitle |
Molecular epidemiology of Vibrio cholerae O1 isolates from Colombia.
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pubmed:affiliation |
Instituto Nacional de Salud, Santafé de Bogota, Colombia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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