Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
16
|
| pubmed:dateCreated |
1997-8-28
|
| pubmed:abstractText |
The expression of multiple classes of voltage-dependent calcium channels (VDCCs) allows neurons to tailor calcium signaling to functionally discrete cellular regions. In the developing hippocampus a central issue is whether the expression of VDCC subtypes plays a role in key phases such as migration and synaptogenesis. Using radioligand binding and immunoblotting, we show that some N-type VDCCs exist before birth, consistent with a role in migration; however, most N-VDCC subunit expression is postnatal, coinciding with synaptogenesis. Immunoprecipitation studies indicate that the increased expression of N-VDCCs in early development occurs without subunit switching because there is no change in the fraction of beta3 subunits in the N-VDCC alpha1B-beta3 heteromers. Fluorescence imaging of cell surface N-VDCCs during this period reveals that N-VDCCs are expressed on somata before dendrites and that this expression is asynchronous between different subfields of the hippocampus (CA3-CA4 before CA1-CA2 and dentate gyrus). Our data argue that N-VDCC expression is an important cue in the genesis of synaptic transmission in discrete hippocampal subfields.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/omega-Conotoxin GVIA
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0270-6474
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6152-64
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9236227-Animals,
pubmed-meshheading:9236227-Antibodies,
pubmed-meshheading:9236227-Antibody Specificity,
pubmed-meshheading:9236227-Calcium Channel Blockers,
pubmed-meshheading:9236227-Calcium Channels,
pubmed-meshheading:9236227-Cerebral Cortex,
pubmed-meshheading:9236227-Female,
pubmed-meshheading:9236227-Gene Expression Regulation, Developmental,
pubmed-meshheading:9236227-Hippocampus,
pubmed-meshheading:9236227-Immunoblotting,
pubmed-meshheading:9236227-Ion Channel Gating,
pubmed-meshheading:9236227-Peptides,
pubmed-meshheading:9236227-Precipitin Tests,
pubmed-meshheading:9236227-Pregnancy,
pubmed-meshheading:9236227-Pyramidal Cells,
pubmed-meshheading:9236227-Rabbits,
pubmed-meshheading:9236227-Radioligand Assay,
pubmed-meshheading:9236227-Rats,
pubmed-meshheading:9236227-Rats, Wistar,
pubmed-meshheading:9236227-omega-Conotoxin GVIA
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
N-Type calcium channels in the developing rat hippocampus: subunit, complex, and regional expression.
|
| pubmed:affiliation |
Playfair Neuroscience Unit, Toronto Hospital Research Institute, Toronto Hospital Western Division, Toronto, Ontario, Canada M5T 2S8.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|