Linked Life Data

9236219

Source:http://linkedlifedata.com/resource/pubmed/id/9236219

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1997-8-28
pubmed:abstractText
GABAergic and glycinergic IPSCs were studied in identified retinal ganglion cells (RGCs) of light-adapted rat retinal slices, using whole-cell recording techniques. GABAergic IPSCs were blocked specifically by SR95531 (3 microM) and bicuculline (3 microM) and glycinergic IPSCs by strychnine (0.3 microM). From 37 RGCs studied, 25 showed exclusively GABAergic IPSCs, 6 presented only glycinergic IPSCs, and 6 showed both. This distribution may result from differences in amacrine cells input rather than from receptor heterogeneity, because both GABA and glycine elicited Cl--selective currents in all RGCs tested. TTX markedly reduced GABAergic IPSCs frequency, whereas glycinergic IPSCs were unaffected. Ca2+-free media, with or without high Mg2+, blocked TTX-resistant GABAergic and glycinergic IPSCs. These results suggest that GABAergic IPSCs in RGCs can be elicited either by Na+-dependent action potentials or by local Ca2+ influx in medium or large dendritic field GABAergic amacrine cells, whereas glycinergic IPSCs are generated by action potential-independent Ca2+ influx in narrow field glycinergic amacrine cells. Both types of IPSCs had fast rise times and biexponential decays, but glycinergic IPSC decay was significantly slower than that of GABAergic IPSCs. An elementary conductance of 54 pS for the glycine-gated channels was estimated from single-channel events, clearly detected in the falling phase of glycinergic IPSCs, and from responses to exogenous glycine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6075-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
GABAergic and glycinergic IPSCs in ganglion cells of rat retinal slices.
pubmed:affiliation
Arbeitsgruppe Zelluläre Neurobiologie, Max-Planck-Institut für biophysikalische Chemie, 37070 Göttingen, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't