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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1997-8-14
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pubmed:abstractText |
There are several indications suggesting that interleukin-1beta (IL-1beta) may play an important role in inflammatory periodontal diseases. We hypothesized that periodontal sites would represent a unique combination of both cellular sources of IL-1beta precursor (pro-IL-1beta) and microbial proteases and proposed that Treponema denticola, a suspected periodontal pathogen, would play a critical role in the inflammatory nature of adult chronic periodontitis by activating pro-IL-1beta. The aim of this study was thus to demonstrate the proteolytic cleavage and activation of the inactive precursor pro-IL-1beta by T. denticola. After incubation of bacterial cells with recombinant pro-IL-1beta, proteolytic cleavage was monitored by Western immunoblotting, and the biological activity of the digestion products was tested in a bioassay. We report here that T. denticola can cleave pro-IL-1beta to yield two fragments with molecular masses of 18 and 19 kDa. Cleavage products showed a dose-dependent biological activity in the thymocyte proliferation bioassay, and this activity was inhibited by anti-IL-1beta neutralizing antibodies. These results suggest that T. denticola may have a proinflammatory role in periodontal diseases.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-1919436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-1999487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-2002430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-2087354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-2156847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-2405268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-2420874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-2787508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-2955410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-2989698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-3013780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-3166451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-3259579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-3271022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-3288634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-3492551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-5033418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-6606682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7152665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7309842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7505322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7613756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7642269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7689226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7727685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7775673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7845238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-7877078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-8046557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-8168936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-8263720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-8315564,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-8803947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-8936322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234775-9673164
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3199-202
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9234775-Animals,
pubmed-meshheading:9234775-Chronic Disease,
pubmed-meshheading:9234775-Interleukin-1,
pubmed-meshheading:9234775-Male,
pubmed-meshheading:9234775-Mice,
pubmed-meshheading:9234775-Mice, Inbred DBA,
pubmed-meshheading:9234775-Peptide Fragments,
pubmed-meshheading:9234775-Periodontitis,
pubmed-meshheading:9234775-Protein Precursors,
pubmed-meshheading:9234775-Treponema
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pubmed:year |
1997
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pubmed:articleTitle |
Activation of the interleukin-1beta precursor by Treponema denticola: a potential role in chronic inflammatory periodontal diseases.
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pubmed:affiliation |
Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Québec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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