Linked Life Data

9234773

Source:http://linkedlifedata.com/resource/pubmed/id/9234773

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1997-8-14
pubmed:abstractText
The induction by intracellular pathogens of interleukin-12 (IL-12) secretion is of particular importance since this cytokine has been shown to be necessary for optimal cell-mediated immune responses. Several recent investigations have suggested that cultured macrophages are a significant source of IL-12 following intracellular infection with pathogens such as Salmonella spp. In an effort to critically evaluate the magnitude of the IL-12 response in cultured macrophages following interaction with Salmonella dublin, enzyme-linked immunosorbent assays specific for the 40- and 70-kDa subunits of IL-12 (IL-12p40 and IL-12p70) and a sensitive bioassay for IL-12p70 were used. Using BALB/c macrophages, S. dublin at various challenge doses was a potent inducer of IL-12p40 secretion (>6,000 pg/10(7) macrophages). However when secretion of IL-12p70 was evaluated, S. dublin did not induce comparable IL-12p70 production (<80 pg/10(7) macrophages) at any time, despite varying the challenge dose of Salmonella. The limited ability of BALB/c (Ity(s)) macrophages to secrete IL-12p70 in response to Salmonella was not a strain-specific phenomenon since similar results were demonstrated for macrophages isolated from CBA/J (Ity(r)) and C3H/HeJ (lipopolysaccharide [LPS]-hyporesponsive) mice. While intracellular infection with Salmonella was not a potent stimulus for IL-12p70 secretion in these mouse strains, macrophages from these mice responded significantly to a stimulus of gamma interferon plus LPS. Taken together these results demonstrate a limited capacity for intracellular Salmonella to stimulate murine macrophage secretion of IL-12p70, despite being a significant stimulus for IL-12p40 secretion. Furthermore, our results suggest that Salmonella-induced IL-12p40 secretion by macrophages is not solely an LPS-mediated event.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-1357073, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-1673147, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-1674604, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-1898913, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-202651, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-2204066, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-2504877, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-381178, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7527811, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7561037, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7602106, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7605994, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7612223, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7650381, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7705395, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7751026, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7807019, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7843232, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7868230, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7901851, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7904381, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7908322, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7927680, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-7968625, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8096238, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8097524, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8098733, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8103745, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8557339, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8557998, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8557999, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8606072, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8606114, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8609419, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8613386, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8780734, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8906830, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8945580, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-8977200, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-9036982, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-9125583, http://linkedlifedata.com/resource/pubmed/commentcorrection/9234773-9127002
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3186-92
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Intracellular Salmonella dublin induces substantial secretion of the 40-kilodalton subunit of interleukin-12 (IL-12) but minimal secretion of IL-12 as a 70-kilodalton protein in murine macrophages.
pubmed:affiliation
Department of Microbiology and Immunology, Tulane University Medical Center, New Orleans, Louisiana 70112, USA. kbost@tmcpop.tmc.tulane.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.