| pubmed-article:9233618 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9233618 | lifeskim:mentions | umls-concept:C0282641 | lld:lifeskim |
| pubmed-article:9233618 | lifeskim:mentions | umls-concept:C0004561 | lld:lifeskim |
| pubmed-article:9233618 | lifeskim:mentions | umls-concept:C0034789 | lld:lifeskim |
| pubmed-article:9233618 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:9233618 | lifeskim:mentions | umls-concept:C1254042 | lld:lifeskim |
| pubmed-article:9233618 | lifeskim:mentions | umls-concept:C1709634 | lld:lifeskim |
| pubmed-article:9233618 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9233618 | pubmed:dateCreated | 1997-8-14 | lld:pubmed |
| pubmed-article:9233618 | pubmed:abstractText | IL-7 supports the proliferation of B cell precursors, but inhibits their maturation to mature surface IgM+ (sIgM+) B cells. This inhibition is thought to occur by direct or indirect down-regulation of recombinase genes, preventing the B cells from undergoing Ig light chain rearrangements. To directly analyze the IL-7 inhibitory effects, we studied B cell development and maturation in B cells bearing a transgenic (Tg) B cell receptor (BCR). We show here that proliferation of Tg B cell precursors is IL-7 dependent both in vivo and in vitro and is comparable to that of non-Tg B cell precursors. Tg B cell precursors grown on stroma and IL-7 expressed sIgM on >90% of the cells, and a large proportion of these cells coexpressed additional maturation markers such as IgD, CD23, CD21, and L-selectin, indicating that IL-7 does not inhibit maturation of Tg B cell precursors. The presence of the Tg inhibited V(D)J recombination in the cultured cells, as very low levels of recombination activating genes 2 (RAG-2) expression and endogenous V-Jkappa DNA rearrangements were found. Expression levels of RAG mRNAs were not significantly changed after removal of IL-7 from the in vitro Tg B cell cultures. In contrast, we found that IL-7 inhibited maturation of non-Tg B cell precursors and that removal of IL-7 resulted in a significant increase in RAG-2 expression and kappa rearrangements, thus allowing the B cells to express sIgM and to mature. These results suggest that IL-7-mediated inhibition of Ig gene rearrangement blocks maturation of B cell precursors and that the presence of Tg BCR efficiently circumvents this inhibition. | lld:pubmed |
| pubmed-article:9233618 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9233618 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9233618 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9233618 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9233618 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9233618 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:9233618 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9233618 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9233618 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9233618 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9233618 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9233618 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9233618 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:9233618 | pubmed:author | pubmed-author:RolineGG | lld:pubmed |
| pubmed-article:9233618 | pubmed:author | pubmed-author:GrabsteinKK | lld:pubmed |
| pubmed-article:9233618 | pubmed:author | pubmed-author:NemazeeDD | lld:pubmed |
| pubmed-article:9233618 | pubmed:author | pubmed-author:MelamedDD | lld:pubmed |
| pubmed-article:9233618 | pubmed:author | pubmed-author:KenchJ AJA | lld:pubmed |
| pubmed-article:9233618 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9233618 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9233618 | pubmed:volume | 159 | lld:pubmed |
| pubmed-article:9233618 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9233618 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9233618 | pubmed:pagination | 1233-9 | lld:pubmed |
| pubmed-article:9233618 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:9233618 | pubmed:meshHeading | pubmed-meshheading:9233618-... | lld:pubmed |
| pubmed-article:9233618 | pubmed:meshHeading | pubmed-meshheading:9233618-... | lld:pubmed |
| pubmed-article:9233618 | pubmed:meshHeading | pubmed-meshheading:9233618-... | lld:pubmed |
| pubmed-article:9233618 | pubmed:meshHeading | pubmed-meshheading:9233618-... | lld:pubmed |
| pubmed-article:9233618 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9233618 | pubmed:articleTitle | A functional B cell receptor transgene allows efficient IL-7-independent maturation of B cell precursors. | lld:pubmed |
| pubmed-article:9233618 | pubmed:affiliation | Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA. | lld:pubmed |
| pubmed-article:9233618 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9233618 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9233618 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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