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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
1997-10-15
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pubmed:abstractText |
In vitro differentiated embryonic stem (ES) cells contain a population which is similar to fetal liver pro/pre-B cells on the basis of cell surface antigens and cytoplasmic expression of immunoglobin heavy chain. This population was purified and transplanted into Rag-1 deficient recipients to characterize its developmental potential in vivo. Following intravenous transfer, these cells rapidly reconstituted the splenic B but not the T cell compartment. Reconstitution was transient, indicating the lack of long-term reconstituting capacity. Similar to fetal liver, B-1 type as well as conventional B cells were generated, accompanied by high serum IgM levels. Intraperitoneal injection generated high numbers of peritoneal B cells, predominately of the B-1a phenotype, with poor splenic repopulation and low serum IgM levels. These observations suggest the emergence of two different B lineage precursor populations during in vitro ES cell differentiation and define a possible role of the microenvironment in directing lymphoid development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0165-2478
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9232439-Animals,
pubmed-meshheading:9232439-Antigens, CD45,
pubmed-meshheading:9232439-Antigens, CD5,
pubmed-meshheading:9232439-B-Lymphocyte Subsets,
pubmed-meshheading:9232439-Cell Differentiation,
pubmed-meshheading:9232439-Cell Line,
pubmed-meshheading:9232439-DNA-Binding Proteins,
pubmed-meshheading:9232439-Female,
pubmed-meshheading:9232439-Genes, RAG-1,
pubmed-meshheading:9232439-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:9232439-Homeodomain Proteins,
pubmed-meshheading:9232439-Immunoglobulin D,
pubmed-meshheading:9232439-Immunoglobulin M,
pubmed-meshheading:9232439-Male,
pubmed-meshheading:9232439-Mice,
pubmed-meshheading:9232439-Mice, Inbred BALB C,
pubmed-meshheading:9232439-Mice, Inbred C57BL,
pubmed-meshheading:9232439-Stem Cells
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pubmed:year |
1997
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pubmed:articleTitle |
Reconstitution of B cell subsets in Rag deficient mice by transplantation of in vitro differentiated embryonic stem cells.
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pubmed:affiliation |
Max-Planck-Institute for Immunobiology, Freiburg, Germany. potocnik@immunbio.mpg.de
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pubmed:publicationType |
Journal Article
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