Linked Life Data

9231798

Source:http://linkedlifedata.com/resource/pubmed/id/9231798

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1997-8-15
pubmed:abstractText
GH appears to play an important metabolic role during late pregnancy and in lactation maintenance. In this study, pregnant (days 8, 15, and 20 of gestation) and postpartum (days 3 and 8 postpartum, including lactating and nonlactating dams) Wistar rats were used to investigate pituitary GH gene expression and hormone secretion, and the potential alterations of the major signals regulating GH secretion and action [somatostatin (SS) and GH-releasing hormone (GHRH), GH receptor (GH-R), and insulin-like growth factor-I (IGF-I)]. GH and SS messenger RNA (mRNA) were quantitated by Northern blot, and both IGF-I and GH-R mRNA were analyzed by the ribonuclease protection assay technique. Pituitary IR-GH content and GH mRNA increased at midpregnancy. IR-GH content was decreased in lactating rats. Plasma GH levels progressively increased during pregnancy, whereas no significant alterations were shown during lactation. Elevated GH levels persisted during lactation. Levels at this time were higher in nonsuckling compared with suckling dams. Liver GH-R mRNA progressively decreased during pregnancy, but it remained unchanged during lactation. Plasma IGF-I and liver IR-IGF-I constantly decreased during pregnancy, and no significant modifications were seen either in suckling or in nonsuckling animals. IGF-I mRNA accumulation in the liver decreased during pregnancy. After delivery, a progressive decrease of liver IGF-I mRNA occurred. At the hypothalamic level, a progressive increase in the IR-SS content was found during pregnancy, with no SS mRNA modification. After delivery, a higher hypothalamic IR-SS content was found in lactating than in nonlactating rats, with no changes in SS mRNA levels. Hypothalamic IR-IGF-I also showed a progressive increase during pregnancy with no significant alterations during lactation. Hypothalamic IR-GHRH presented a nonsignificant mild increase during pregnancy with no modifications during lactation. In the pituitary, IR-IGF-I content progressively increased during gestation, reaching its highest concentration at day 20. During lactation, pituitary IGF-I did not change. In summary, our data show that the mechanisms of the increase in plasma GH levels occurring during pregnancy include an increase in GH gene expression in the pituitary, a decrease in SS secretion from the hypothalamus, an increase in IR-IGF-I content in the hypothalamus and in the pituitary, and a significant decrease in circulating IGF-I. Plasma and liver IR-IGF-I and IGF-I mRNA in the liver decreased throughout gestation due to a lower GH-R gene expression in the liver. This state of GH resistance with a higher GH/IGF-I ratio could be important in providing supplementary nutrients to the fetus. During lactation, GH and its regulatory machinery did not show important modifications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3435-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9231798-Animals, pubmed-meshheading:9231798-Blotting, Western, pubmed-meshheading:9231798-Estrogens, pubmed-meshheading:9231798-Female, pubmed-meshheading:9231798-Gene Expression Regulation, pubmed-meshheading:9231798-Growth Hormone, pubmed-meshheading:9231798-Growth Hormone-Releasing Hormone, pubmed-meshheading:9231798-Hypothalamus, pubmed-meshheading:9231798-Insulin-Like Growth Factor I, pubmed-meshheading:9231798-Lactation, pubmed-meshheading:9231798-Liver, pubmed-meshheading:9231798-Pituitary Gland, pubmed-meshheading:9231798-Pregnancy, pubmed-meshheading:9231798-Pregnancy, Animal, pubmed-meshheading:9231798-Progesterone, pubmed-meshheading:9231798-RNA, Messenger, pubmed-meshheading:9231798-Rats, pubmed-meshheading:9231798-Rats, Wistar, pubmed-meshheading:9231798-Receptors, Somatotropin, pubmed-meshheading:9231798-Somatostatin
pubmed:year
1997
pubmed:articleTitle
Regulation of growth hormone (GH) gene expression and secretion during pregnancy and lactation in the rat: role of insulin-like growth factor-I, somatostatin, and GH-releasing hormone.
pubmed:affiliation
Endocrinology Services, Centro de Investigación Clínica, Instituto Carlos III, Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't