Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1980-1-24
pubmed:abstractText
By using the jejunal tract of rat and hamster intestine, net fluid transintestinal transport in the everted sac incubated in vitro at 28 degrees C and at 38 degrees C has been determined. In the scraped mucosa wet weight/dry weight ratio, cell water, sodium and potassium concentration have been detected in vivo and in vitro, throughout the incubation time, at the two different temperatures. Under all these conditions ATP, ADP and AMP levels have been determined in total intestine and in scraped mucosa. In rat jejunum incubated in vitro at 38 degrees C transintestinal fluid transport continuously decreases during 1-h experiment; in the same time the enterocyte gains sodium, dilutes cell potassium and swells, whereas its energy charge is dramatically reduced. All these parameters are constant in rat jejunum incubated at 28 degrees C and in the hamster incubated both at 28 degrees C and at 38 degrees C throughout the experiment. An inadequate diffusion of oxygen into the enterocyte, could tentatively explain experimental results obtained on rat jejunum at 38 degrees C. Under all tested conditions, energy charge and intracellular potassium are lower in conditions in vitro than in those in vivo; the contrary happens for cell sodium and swelling. The oxygenation of the intestine in vitro, lower than the one in vivo, could explain the different behaviour found in the two experimental conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0003-9799
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-86
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
The rat and hamster jejunum during transintestinal transport in vitro.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro