Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
30
pubmed:dateCreated
1997-9-9
pubmed:abstractText
There are three isoforms of the sarcoplasmic reticulum Ca2+-ATPase; they are known as SERCA1, SERCA2, and SERCA3. Phospholamban is present in tissues that express the SERCA2 isoform and is an inhibitor of the affinity of SERCA2 for calcium. In vitro reconstitution and cell culture expression studies have shown that phospholamban can also regulate SERCA1, the fast-twitch skeletal muscle isoform. To determine whether regulation of SERCA1 by phospholamban can be of physiological relevance, we generated transgenic mice that ectopically express phospholamban in fast-twitch skeletal muscle, a tissue normally devoid of phospholamban. Ectopic expression of phospholamban was associated with a decrease in the affinity of SERCA1 for calcium. Assessment of isometric twitch contractions of intact fast-twitch skeletal muscles revealed depressed rates of relaxation in transgenic mice compared with wild-type cohorts. Furthermore, the prolongation of muscle relaxation appeared to correlate with the levels of phospholamban expressed in two transgenic mouse lines. These findings indicate that ectopic expression of phospholamban in fast-twitch skeletal muscle is associated with inhibition of SERCA1 activity and decreased relaxation rates of this muscle.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18862-8
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:9228063-Adrenergic beta-Agonists, pubmed-meshheading:9228063-Animals, pubmed-meshheading:9228063-Biological Transport, pubmed-meshheading:9228063-Blotting, Western, pubmed-meshheading:9228063-Calcium, pubmed-meshheading:9228063-Calcium-Binding Proteins, pubmed-meshheading:9228063-Calcium-Transporting ATPases, pubmed-meshheading:9228063-Female, pubmed-meshheading:9228063-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:9228063-Gene Expression, pubmed-meshheading:9228063-Immune Sera, pubmed-meshheading:9228063-Isoenzymes, pubmed-meshheading:9228063-Isometric Contraction, pubmed-meshheading:9228063-Isoproterenol, pubmed-meshheading:9228063-Male, pubmed-meshheading:9228063-Mice, pubmed-meshheading:9228063-Mice, Transgenic, pubmed-meshheading:9228063-Muscle, Skeletal, pubmed-meshheading:9228063-Muscle Fibers, Fast-Twitch, pubmed-meshheading:9228063-Muscle Relaxation, pubmed-meshheading:9228063-Sarcoplasmic Reticulum, pubmed-meshheading:9228063-Transgenes
pubmed:year
1997
pubmed:articleTitle
Ectopic expression of phospholamban in fast-twitch skeletal muscle alters sarcoplasmic reticulum Ca2+ transport and muscle relaxation.
pubmed:affiliation
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.