Linked Life Data

9220968

Source:http://linkedlifedata.com/resource/pubmed/id/9220968

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
29
pubmed:dateCreated
1997-9-8
pubmed:abstractText
BP180 is a glycoprotein constituent of the epidermal anchoring complex and a major antigenic target of autoantibodies associated with bullous pemphigoid, a blistering skin disease. The C-terminal extracellular domain of BP180 contains 15 domains composed of Gly-X-Y tandem repeats, which are predicted to form collagen-like triple helices. To facilitate the structural analysis of this protein, the extracellular region of human BP180 was expressed as a secreted protein (sec180e) in transiently transfected COS-1 cells. Gel filtration and sedimentation analyses demonstrated that sec180e exists in two forms: a globular monomeric form and a high-molecular mass multimeric form with an elongated conformation. Pulse-chase and cross-linking experiments established that the sec180e complex is a stable homotrimeric structure which assembles prior to secretion from the cell. On the basis of its calculated molecular mass, the oligomeric state of the sec180e complex is 3.25. With a Stokes radius of 13.6 nm, a sedimentation coefficent of 6.5 S, and a frictional ratio of 3.01, the sec180e protein appears to be highly extended (length to width ratio is between 52 and 60), yet is more flexible than a rigid rod. BP180 isolated from human epidermis was also shown to exist in a high-molecular mass complex which, like sec180e and other collagenous proteins, is SDS-stable but heat-labile. These findings strongly suggest that the BP180 ectodomain exists as an elongate, flexible homotrimer. This trimerization is likely to result from the formation of stable collagen triple-helical and coiled-coil type structures and does not depend upon the presence of the cytoplasmic or transmembrane domains of this protein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8821-30
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
A recombinant form of the human BP180 ectodomain forms a collagen-like homotrimeric complex.
pubmed:affiliation
Department of Dermatology, Medical College of Wisconsin, and Veterans Affairs' Medical Center, Milwaukee, Wisconsin 53226, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.