Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1997-9-29
|
pubmed:abstractText |
The unicellular eukaryotic dinoflagellates shed their flagella and form a new pellicle cyst wall in response to environmental stress. This encystment process can also be induced by indoleamines such as melatonin and 5-methoxytryptamine. To decipher the complex signaling events which lead to encystment, we have investigated the functional roles of Ca2+ and inositol phosphates in indoleamine-induced encystment of the dinoflagellates Alexandrium catenella and Crypthecodinium cohnii. Pretreatment with EGTA, but not with EDTA, effectively blocked the indoleamine-induced encystment of A. catenella in a dose-dependent manner. Conversely, agents that facilitate the influx of Ca2+ (Bay K 8644, A23187 and ionomycin) dose-dependently induced encystment of A. catenella. Endoplasmic Ca2+-ATPase inhibitors such as thapsigargin and the peptide toxin melittin also induced encystment of A. catenella. These results suggest that an elevation of intracellular [Ca2+] may be involved in the encystment response. In terms of the regulation of phospholipase C, melatonin dose- and time-dependently stimulated the formation of inositol phosphates in C. cohnii. The rank order of potency for several indoleamines to stimulate inositol phosphates formation was 2-iodomelatonin > 5-methoxytryptamine > or = melatonin >> N-acetylserotonin > 5-hydroxytryptamine. This rank order was the same as for the indoleamine-induced encystment of C. cohnii as previously reported. Our results indicate that indoleamine-induced activation of phospholipase C and elevation of intracellular [Ca2+] may be proximal steps in the signal transduction pathway leading to encystment in dinoflagellates. Moreover, this is the first demonstration of the possible involvement of Ca2+ and inositol phosphates as second messengers in dinoflagellates.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-Methoxytryptamine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Melatonin,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0021-9533
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
110 ( Pt 12)
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1387-93
|
pubmed:dateRevised |
2007-11-15
|
pubmed:meshHeading |
pubmed-meshheading:9217324-5-Methoxytryptamine,
pubmed-meshheading:9217324-Animals,
pubmed-meshheading:9217324-Calcium,
pubmed-meshheading:9217324-Cysts,
pubmed-meshheading:9217324-Dinoflagellida,
pubmed-meshheading:9217324-Dose-Response Relationship, Drug,
pubmed-meshheading:9217324-Inositol Phosphates,
pubmed-meshheading:9217324-Melatonin,
pubmed-meshheading:9217324-Signal Transduction,
pubmed-meshheading:9217324-Time Factors,
pubmed-meshheading:9217324-Type C Phospholipases
|
pubmed:year |
1997
|
pubmed:articleTitle |
Calcium ion dependency and the role of inositol phosphates in melatonin-induced encystment of dinoflagellates.
|
pubmed:affiliation |
Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|