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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-10-3
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pubmed:databankReference |
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pubmed:abstractText |
We have solved the crystal structure of the Fab fragment of NMC-4, a mouse monoclonal antibody that binds to the A1 domain of von Willebrand factor (vWF). Two Asp and three Tyr residues in the complementarity determining regions 1 and 3 of the heavy chain exhibited a spatial orientation suggestive of a dominant role in establishing contact with the antigen. A cluster of Asp and Tyr residues occurs also in a region of the platelet glycoprotein (GP) Ib alpha amino terminal domain known to be critically involved in vWF binding. Thus, the structural information obtained with NMC-4 may prove relevant to understand the stereochemical bases of the GP Ib alpha-vWF interaction essential for thrombus formation at sites of vascular lesion.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1079-9796
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
123-34
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pubmed:dateRevised |
2011-9-22
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pubmed:meshHeading |
pubmed-meshheading:9215757-Amino Acid Sequence,
pubmed-meshheading:9215757-Animals,
pubmed-meshheading:9215757-Antibodies, Monoclonal,
pubmed-meshheading:9215757-Base Sequence,
pubmed-meshheading:9215757-Cloning, Molecular,
pubmed-meshheading:9215757-Crystallography, X-Ray,
pubmed-meshheading:9215757-Immunoglobulin Fab Fragments,
pubmed-meshheading:9215757-Immunoglobulin Heavy Chains,
pubmed-meshheading:9215757-Immunoglobulin Light Chains,
pubmed-meshheading:9215757-Mice,
pubmed-meshheading:9215757-Models, Molecular,
pubmed-meshheading:9215757-Molecular Sequence Data,
pubmed-meshheading:9215757-Protein Conformation,
pubmed-meshheading:9215757-Recombinant Proteins,
pubmed-meshheading:9215757-von Willebrand Factor
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pubmed:year |
1997
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pubmed:articleTitle |
Crystal structure of NMC-4 fab anti-von Willebrand factor A1 domain.
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pubmed:affiliation |
Roon Research Center for Arteriosclerosis and Thrombosis, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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