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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-8-21
|
| pubmed:abstractText |
Intraluminal delivery of antisense oligonucleotides to c-myb was assessed following balloon angioplasty in swine peripheral arteries. Successful delivery and intramural persistence of oligonucleotide for over 24 h were demonstrated following angioplasty with hydrogel balloons coated with 32P-labeled antisense. Delivery of fluorescein-labeled antisense demonstrated further localization within the arterial media and intracellularly. Preliminary in vitro studies demonstrated the feasibility of inhibition of porcine lymphocyte proliferation using the murine antisense to c-myb. Twelve iliac or carotid arteries underwent angioplasty with antisense-coated balloons, while the contralateral vessels underwent angioplasty with the same-sized balloons coated with the complementary sense strand. Six to seven days later, dilated arterial segments were surgically isolated. In 10 of 12 vessel pairs, antisense-treated vessels demonstrated less cellular proliferation than did contralateral sense-treated vessels, as assessed by quantitative immunohistochemical staining of proliferating cell nuclear antigen, and smooth muscle cell proliferation was reduced 18% in antisense-treated vessels compared to the contralateral sense-treated vessels (PCNA-positive nuclear area: 7.7 +/- 4.9% vs. 9.3 +/- 5.2%, P < 0.04)-intraluminal delivery of antisense oligonucleotides to c-myb is feasible with a catheter-based system and may reduce smooth muscle cell proliferation following arterial injury.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Proliferating Cell Nuclear Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myb,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0098-6569
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
41
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
232-40
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:9213020-Angioplasty, Balloon, Coronary,
pubmed-meshheading:9213020-Animals,
pubmed-meshheading:9213020-Autoradiography,
pubmed-meshheading:9213020-Cell Division,
pubmed-meshheading:9213020-Coronary Disease,
pubmed-meshheading:9213020-Coronary Vessels,
pubmed-meshheading:9213020-Drug Delivery Systems,
pubmed-meshheading:9213020-Feasibility Studies,
pubmed-meshheading:9213020-Female,
pubmed-meshheading:9213020-Lymphocyte Activation,
pubmed-meshheading:9213020-Mice,
pubmed-meshheading:9213020-Microscopy, Fluorescence,
pubmed-meshheading:9213020-Muscle, Smooth, Vascular,
pubmed-meshheading:9213020-Oligonucleotides, Antisense,
pubmed-meshheading:9213020-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:9213020-Proto-Oncogene Proteins,
pubmed-meshheading:9213020-Proto-Oncogene Proteins c-myb,
pubmed-meshheading:9213020-Swine,
pubmed-meshheading:9213020-Trans-Activators
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Local delivery of c-myb antisense oligonucleotides during balloon angioplasty.
|
| pubmed:affiliation |
Department of Internal Medicine and Cardiology, Hartford Hospital, Connecticut, USA.
|
| pubmed:publicationType |
Journal Article
|