Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-9-11
pubmed:abstractText
To understand whether osteogenesis imperfecta (OI) could result from defective differentiation of osteoprogenitor cells, we investigated the osteogenic potential of bone marrow stromal cells from a mouse model of human OI (oim). Bone marrow was flushed from the femurs and tibias of oim and normal littermates using a syringe with Dulbecco's modified Eagle's medium, and cells were allowed to adhere to flasks. Adherent cells were trypsinized and passaged weekly at a 1:4 split. The established stromal cells were assessed for collagen synthesis, alkaline phosphatase, and osteocalcin production in the presence or absence of rhBMP-2. The stromal cells were also assessed for mineralization by Von-Kossa staining and for exogenous gene transfer using adeno-lacZ and a retroviral vector. The bone marrow stromal cells from oim mice synthesized alpha 1(I) homotrimers as expected, whereas the stromal cells from the normal littermates synthesized alpha 1(I)2 alpha 2(I) heterotrimers. The bone marrow stromal cells exhibited low levels of alkaline phosphatase activity under basal conditions: upon treatment with rhBMP-2, the level of the alkaline phosphatase activity increased approximately 40-fold. Cytochemical staining of the cells confirmed the expression of alkaline phosphatase by the oim stromal cells and its augmentation by rhBMP-2. Osteocalcin production in the stromal cells was also enhanced approximately threefold by rhBMP-2. oim stromal cells grown in the presence of beta-glycerophosphate and ascorbic acid demonstrated Von-Kossa-positive solid deposits after 3 weeks in culture. Ten days after infection with adeno-lacZ, approximately 70% of oim stromal cells expressed the transgene product, and after infection with a retrovirus, approximately 20% of the cells expressed the transgene. These data indicate that bone marrow stromal cells, have osteogenic potential, and also the potential to be transduced with exogenous genes. Under basal conditions, however, the stromal cells from oim mice exhibited significantly lower levels of alkaline phosphatase activity than their normal littermates.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
8756-3282
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7-15
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9213002-Alkaline Phosphatase, pubmed-meshheading:9213002-Animals, pubmed-meshheading:9213002-Bone Marrow, pubmed-meshheading:9213002-Bone Marrow Cells, pubmed-meshheading:9213002-Bone Morphogenetic Protein 2, pubmed-meshheading:9213002-Bone Morphogenetic Proteins, pubmed-meshheading:9213002-Calcinosis, pubmed-meshheading:9213002-Cell Adhesion, pubmed-meshheading:9213002-Cells, Cultured, pubmed-meshheading:9213002-Collagen, pubmed-meshheading:9213002-Disease Models, Animal, pubmed-meshheading:9213002-Femur, pubmed-meshheading:9213002-Gene Expression Regulation, Viral, pubmed-meshheading:9213002-Humans, pubmed-meshheading:9213002-Lac Operon, pubmed-meshheading:9213002-Mice, pubmed-meshheading:9213002-Osteocalcin, pubmed-meshheading:9213002-Osteogenesis, pubmed-meshheading:9213002-Osteogenesis Imperfecta, pubmed-meshheading:9213002-Polymers, pubmed-meshheading:9213002-Recombinant Proteins, pubmed-meshheading:9213002-Retroviridae Infections, pubmed-meshheading:9213002-Signal Transduction, pubmed-meshheading:9213002-Stromal Cells, pubmed-meshheading:9213002-Tibia, pubmed-meshheading:9213002-Transforming Growth Factor beta
pubmed:year
1997
pubmed:articleTitle
Effect of rhBMP-2 on the osteogenic potential of bone marrow stromal cells from an osteogenesis imperfecta mouse (oim).
pubmed:affiliation
Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't