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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-7-23
|
| pubmed:abstractText |
Analysis by polymerase chain reaction (PCR) and serological studies have demonstrated a close association between the novel human herpes virus, Kaposi's sarcoma-associated herpes virus (KSHV) or human herpes virus-8 (HHV-8) and the development of Kaposi's sarcoma (KS). To clarify the role of HHV-8 in KS pathogenesis, we investigated at the cellular level by in situ hybridization the expression of a recently described 0.7-kb HHV-8-encoded mRNA (T0.7 mRNA) in KS tissues of different epidemiological origin (AIDS-KS, African endemic KS and classical KS). The T0.7 mRNA likely encodes a small membrane protein, supposedly expressed in latently HHV-8-infected cells. Indeed, we detected T0.7 mRNA in virtually all cells of the cell line BCBL-1 established from a body cavity-based lymphoma (BCBL) and latently infected with HHV-8. In all KS biopsies examined, independent of their epidemiological type, the late-stage (nodular) KS tissues showed a high level of T0.7 mRNA expression in typical KS spindle cells but also in endothelial cells lining blood vessels, indicating latent HHV-8 infection of these cells. The presence of T0.7-expressing cells was restricted to KS tumor tissue and therefore appears to indicate an important role of latent HHV-8 infection in KS pathogenesis.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0020-7136
|
| pubmed:author |
pubmed-author:AscherlGG,
pubmed-author:BiberfeldPP,
pubmed-author:BlasigCC,
pubmed-author:BrockmeyerN HNH,
pubmed-author:CornaliEE,
pubmed-author:EkmanMM,
pubmed-author:EsserSS,
pubmed-author:HohenadlCC,
pubmed-author:KaayaE EEE,
pubmed-author:NeipelFF,
pubmed-author:SchreierAA,
pubmed-author:StürzlMM,
pubmed-author:TschachlerEE
|
| pubmed:issnType |
Print
|
| pubmed:day |
3
|
| pubmed:volume |
72
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
68-71
|
| pubmed:dateRevised |
2007-7-24
|
| pubmed:meshHeading |
pubmed-meshheading:9212225-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:9212225-Africa,
pubmed-meshheading:9212225-Biopsy,
pubmed-meshheading:9212225-Epithelium,
pubmed-meshheading:9212225-Herpesvirus 8, Human,
pubmed-meshheading:9212225-Humans,
pubmed-meshheading:9212225-In Situ Hybridization,
pubmed-meshheading:9212225-Polymerase Chain Reaction,
pubmed-meshheading:9212225-RNA, Viral,
pubmed-meshheading:9212225-Sarcoma, Kaposi,
pubmed-meshheading:9212225-Virus Latency
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Expression of HHV-8 latency-associated T0.7 RNA in spindle cells and endothelial cells of AIDS-associated, classical and African Kaposi's sarcoma.
|
| pubmed:affiliation |
Abteilung Virusforschung, Max-Planck-Institut für Biochemie, Martinsried, Germany. STUERZL@Biochem.mpg.de
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|