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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1997-9-2
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pubmed:abstractText |
A GnRH-expressing neuronal cell line (NLT) was used to determine whether insulin-like growth factor I (IGF-I) regulates GnRH gene expression. A receptor-binding assay demonstrated the expression of IGF-I receptors on NLT cells. Activation of IGF-I receptors induced the Ras/Raf-1/mitogen-activated protein kinase pathway and increased c-fos expression. NLT cells treated with IGF-I underwent cell proliferation and exhibited a growth-independent increase in mouse GnRH mRNA expression. In cells transfected with DNA constructs containing the human GnRH promoter, which includes a consensus AP-1 binding site fused to the luciferase reporter gene, a significant increase in reporter activities was induced by IGF-I, whereas mutation of this AP-1 site significantly reduced IGF-I-induced promoter activation. These results demonstrate that IGF-I serves as an important signal in the regulation of both human and rodent GnRH gene expression.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Gonadotropin-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, IGF Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0888-8809
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1145-55
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9212061-Animals,
pubmed-meshheading:9212061-Binding Sites,
pubmed-meshheading:9212061-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:9212061-Cell Division,
pubmed-meshheading:9212061-Cell Line,
pubmed-meshheading:9212061-Enzyme Activation,
pubmed-meshheading:9212061-Gonadotropin-Releasing Hormone,
pubmed-meshheading:9212061-Humans,
pubmed-meshheading:9212061-Insulin-Like Growth Factor I,
pubmed-meshheading:9212061-Mice,
pubmed-meshheading:9212061-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:9212061-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:9212061-Mitogen-Activated Protein Kinases,
pubmed-meshheading:9212061-Neurons,
pubmed-meshheading:9212061-Protein-Tyrosine Kinases,
pubmed-meshheading:9212061-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:9212061-RNA, Messenger,
pubmed-meshheading:9212061-Receptor, IGF Type 1,
pubmed-meshheading:9212061-Signal Transduction,
pubmed-meshheading:9212061-Transcription, Genetic,
pubmed-meshheading:9212061-Transcription Factor AP-1
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pubmed:year |
1997
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pubmed:articleTitle |
Regulation of gonadotropin-releasing hormone (GnRH) gene expression by insulin-like growth factor I in a cultured GnRH-expressing neuronal cell line.
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pubmed:affiliation |
Division of Endocrinology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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