Linked Life Data

9211906

Source:http://linkedlifedata.com/resource/pubmed/id/9211906

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
1997-8-14
pubmed:abstractText
In mammals, plasma concentrations of amino acids are affected by nutritional or pathological conditions. Here we examined the role of amino acid limitation in regulating the expression of CHOP, a CCAAT/enhancer binding protein (C/EBP)-related gene. CHOP protein is capable of interacting with other C/EBPs to modify their DNA binding activities and may function as a negative regulator of these transcription factors. Our data show that leucine limitation in human cell lines leads to induction of CHOP mRNA and protein in a dose-dependent manner. CHOP mRNA induction is rapidly reversed by leucine replenishment. Elevated mRNA levels result from both an increase in the rate of CHOP transcription and an increase in the CHOP mRNA stability. Using a transient expression assay, we show that a promoter fragment, when linked to a reporter gene, is sufficient to mediate the regulation of CHOP expression by leucine starvation in HeLa cells. In addition, we found that decreasing amino acid concentration by itself can induce CHOP expression independently of a cellular stress due to protein synthesis inhibition. Moreover, CHOP expression is induced at leucine concentrations in the range of those observed in blood of protein-restricted animals suggesting that amino acids can participate, in concert with hormones, in the regulation of gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17588-93
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9211906-CCAAT-Enhancer-Binding Proteins, pubmed-meshheading:9211906-Cells, Cultured, pubmed-meshheading:9211906-DNA-Binding Proteins, pubmed-meshheading:9211906-Dactinomycin, pubmed-meshheading:9211906-Gene Expression, pubmed-meshheading:9211906-Glucose, pubmed-meshheading:9211906-HeLa Cells, pubmed-meshheading:9211906-Humans, pubmed-meshheading:9211906-Leucine, pubmed-meshheading:9211906-Nuclear Proteins, pubmed-meshheading:9211906-Promoter Regions, Genetic, pubmed-meshheading:9211906-Protein Processing, Post-Translational, pubmed-meshheading:9211906-Protein Synthesis Inhibitors, pubmed-meshheading:9211906-RNA, Messenger, pubmed-meshheading:9211906-Transcription, Genetic, pubmed-meshheading:9211906-Transcription Factor CHOP, pubmed-meshheading:9211906-Transcription Factors
pubmed:year
1997
pubmed:articleTitle
Amino acid limitation induces expression of CHOP, a CCAAT/enhancer binding protein-related gene, at both transcriptional and post-transcriptional levels.
pubmed:affiliation
Unité de Nutrition Cellulaire et Moléculaire, INRA de Theix, 63122 Saint Genès Champanelle, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't