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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
28
|
| pubmed:dateCreated |
1997-8-14
|
| pubmed:abstractText |
Glucose-dependent insulinotropic polypeptide (GIP) is a 42-amino acid gastrointestinal regulatory peptide that, in the presence of glucose, stimulates insulin secretion. GIP is expressed in K cells of the small intestine and in cells of the submandibular salivary gland. Using a rat GIP cDNA as a specific probe, we screened a number of established cell lines for the expression of GIP mRNA. STC-1 cells, a cell line derived from a mouse neuroendocrine tumor, were found to express high levels of GIP mRNA. GIP-specific transcripts were not detected in other cell lines tested, which included cells of intestinal, salivary, and endocrine origin. Analysis of GIP-luciferase fusions identified two promoters, a distal and a proximal promoter, upstream of the translation initiation codon for GIP. The distal promoter, located upstream of position +1, corresponds to the principal promoter of the GIP gene and can promote cell-specific transcription. Sequential deletion and site-directed mutational analysis of the distal promoter demonstrated that the sequence between -193 and -182 determines cell-specific expression of GIP. Contained in this region is a consensus GATA motif, suggesting that a member of the GATA family of DNA-binding proteins is involved in the cell-specific regulation of the GIP gene.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
11
|
| pubmed:volume |
272
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
17438-43
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9211887-Animals,
pubmed-meshheading:9211887-Base Sequence,
pubmed-meshheading:9211887-Blotting, Northern,
pubmed-meshheading:9211887-Carcinoma, Neuroendocrine,
pubmed-meshheading:9211887-Cloning, Molecular,
pubmed-meshheading:9211887-DNA Mutational Analysis,
pubmed-meshheading:9211887-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9211887-Gastric Inhibitory Polypeptide,
pubmed-meshheading:9211887-Intestinal Mucosa,
pubmed-meshheading:9211887-Male,
pubmed-meshheading:9211887-Mice,
pubmed-meshheading:9211887-Molecular Sequence Data,
pubmed-meshheading:9211887-Mutagenesis, Site-Directed,
pubmed-meshheading:9211887-Rats,
pubmed-meshheading:9211887-Rats, Sprague-Dawley,
pubmed-meshheading:9211887-Restriction Mapping,
pubmed-meshheading:9211887-Transcription, Genetic,
pubmed-meshheading:9211887-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Cell-specific expression of the glucose-dependent insulinotropic polypeptide gene in a mouse neuroendocrine tumor cell line.
|
| pubmed:affiliation |
Section of Gastroenterology, Boston University School of Medicine and Boston Medical Center, Boston, Massachusetts 02118, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|