Linked Life Data

9211517

Source:http://linkedlifedata.com/resource/pubmed/id/9211517

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-10-1
pubmed:abstractText
Incubation for 24 h in culture medium containing 50 mM adenosine triphosphate (ATP) produces distinct alterations in the ultrastructure of Trypanosoma cruzi epimastigotes, most obvious of which is the formation of large membrane-bound vacuoles in the cytosol. These vacuoles become positive following exposure to the macromolecule horseradish peroxidase (HRP). After a 20-min chase in phosphate-buffered saline (PBS) the HRP-positive vacuoles begin to separate into discrete structures such that after a 60-min chase, obvious reservosomes are identifiable. It is hypothesized that extracellular ATP causes increased permeability of the epimastigote's plasma membrane, resulting in ionic fluxes that, in turn, interfere with the normal formation of reservosomes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0932-0113
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
624-6
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
The in vitro effects of extracellular adenosine triphosphate on the ultrastructure of Trypanosoma cruzi epimastigotes.
pubmed:affiliation
Department of Biology, Vanderbilt University, Nashville, TN 37235, USA. bogitsbj@ctrvax.vanderbilt.edu
pubmed:publicationType
Journal Article