Linked Life Data

9211230

Source:http://linkedlifedata.com/resource/pubmed/id/9211230

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-8-22
pubmed:abstractText
Cowdria ruminantium is the etiologic agent of heartwater, a tick-transmitted foreign animal disease with considerable potential for entrance into the USA. A competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect serologic responses to C. ruminantium infection. The cELISA utilized a recombinant form of the C. ruminantium major antigenic protein (MAP-1) as the antigen and an anti-MAP-1 monoclonal antibody as the competing indicator reagent. Experimental antisera to C. ruminantium and a wide variety of related ehrlichial organisms were used to evaluate cELISA reactivity. Only sera against C. ruminantium, Ehrlichia canis, E. chaffeensis, and a recently discovered cervine ehrlichia-like organism reacted positively in the cELISA. Specificity of the cELISA was > or = 99.5% in a survey of 1,774 southeastern US and Puerto Rican slaughter cattle sera but was only 85% in a group of 79 hunter-killed white-tailed deer (Odocoileus virginianus) from the southeastern USA. Reference true-positive and cELISA false-positive sera were further analyzed by end point titrations using the cELISA and by indirect fluorescent antibody (IFA) tests for reactivity with C. ruminantium, E. canis, and E. chaffeensis antigens. True heartwater-positive sera were significantly more reactive using the cELISA and C. ruminantium IFA procedures (P < 0.05), whereas false-positive sera were significantly more reactive with the antigens used in the E. chaffeensis IFA procedure (P < 0.05). A group of sera from 210 field-origin ruminants residing on known or potentially heartwater-endemic Caribbean islands revealed a substantial (12.4%) prevalence of cELISA-positive specimens. The cELISA is a relatively specific serodiagnostic test for heartwater in cattle and could be used to monitor for possible introduction of the disease into the USA. The cELISA may also be an excellent tool for monitoring the success of an ongoing Caribbean Amblyomma tick eradication program designed to eliminate the biological vector responsible for the perpetuation and spread of this dangerous foreign animal disease.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1040-6387
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
130-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9211230-Animals, pubmed-meshheading:9211230-Antibodies, Monoclonal, pubmed-meshheading:9211230-Antibody Specificity, pubmed-meshheading:9211230-Antigens, Bacterial, pubmed-meshheading:9211230-Bacterial Outer Membrane Proteins, pubmed-meshheading:9211230-Cattle, pubmed-meshheading:9211230-Deer, pubmed-meshheading:9211230-Ehrlichia, pubmed-meshheading:9211230-Ehrlichia ruminantium, pubmed-meshheading:9211230-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:9211230-False Positive Reactions, pubmed-meshheading:9211230-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:9211230-Goats, pubmed-meshheading:9211230-Heartwater Disease, pubmed-meshheading:9211230-Prevalence, pubmed-meshheading:9211230-Recombinant Proteins, pubmed-meshheading:9211230-Reproducibility of Results, pubmed-meshheading:9211230-Serologic Tests, pubmed-meshheading:9211230-Sheep, pubmed-meshheading:9211230-United States
pubmed:year
1997
pubmed:articleTitle
Development and evaluation of a recombinant antigen, monoclonal antibody-based competitive ELISA for heartwater serodiagnosis.
pubmed:affiliation
National Veterinary Services Laboratories, US Department of Agriculture, Ames, IA 50010, USA.
pubmed:publicationType
Journal Article, Comparative Study