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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-7-28
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pubmed:abstractText |
The binding isotherm to cellulose of cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium has been compared with that of cellobiohydrolase 1 (CBH 1) from Trichoderma reesei. CDH binds more strongly but more sparsely to cellulose than does CBH 1. In a classical Scatchard analysis, a better fit to a one-site binding model was obtained for CDH than for CBH 1. The binding of both enzymes decreased in the presence of ethylene glycol, increased in the presence of ammonium sulphate and was unaffected by sodium chloride. Attempts to localize the cellulose-binding site on CDH have also been made by exposing enzymically digested CDH to cellulose and isolating the cellulose-bound peptides. The results suggest that the cellulose-binding site is located internally in the amino acid sequence of CDH.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210407-1154954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9210407-1375311,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9210407-2554967,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9210407-8919793
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
324 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
833-8
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:9210407-Amino Acid Sequence,
pubmed-meshheading:9210407-Basidiomycota,
pubmed-meshheading:9210407-Carbohydrate Dehydrogenases,
pubmed-meshheading:9210407-Cellulase,
pubmed-meshheading:9210407-Cellulose,
pubmed-meshheading:9210407-Cellulose 1,4-beta-Cellobiosidase,
pubmed-meshheading:9210407-Kinetics,
pubmed-meshheading:9210407-Molecular Sequence Data,
pubmed-meshheading:9210407-Protein Binding,
pubmed-meshheading:9210407-Sequence Homology, Amino Acid
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pubmed:year |
1997
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pubmed:articleTitle |
Studies of cellulose binding by cellobiose dehydrogenase and a comparison with cellobiohydrolase 1.
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pubmed:affiliation |
Department of Biochemistry, University of Uppsala, Biomedical Center, Box 576, S-751 23 Uppsala, Sweden.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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