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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1997-8-7
|
| pubmed:abstractText |
Interleukin-5 (IL-5) is produced by T lymphocytes and known to support B cell growth and eosinophilic differentiation of the progenitor cells. Using ATL-16T cells which express IL-5 mRNA, we have identified a region, within the human IL-5 gene promoter, that regulates IL-5 gene transcription. This cis-acting sequence contains the core binding motif, (A/T)GATA(A/G), for GATA-binding family proteins and thus suggests the involvement of these family members. In this report, we describe the cloning of human GATA-4 (hGATA-4) and show that hGATA-4 selectively interacts with the -70 GATA site within the IL-5 proximal promoter region. By promoter deletion and mutation analyses, we established this region as a positive regulatory element. Cotransfection experiments revealed that both hGATA-4 and PMA/A23187 stimulation are necessary for the IL-5 promoter activation. The requirement of another regulatory element called CLE0, which lies downstream of the -70 GATA site, was also demonstrated. ATL-16T cells express mRNA of three GATA-binding proteins, hGATA-2, hGATA-3 and hGATA-4, and each of them has a potential to bind to the consensus (A/T)GATA(G/ A) motif. However, using ATL-16T nuclear extract, we demonstrated that GATA-4 is the only GATA-binding protein that forms specific DNA-protein complex with the -70 GATA site. The electrophoretic mobility shift assay with extracts of COS cells expressing GATA-binding proteins showed that GATA-4 has the highest binding affinity to the -70 GATA site among the three GATA-binding proteins. When the transactivation ability was compared among the three, GATA-4 showed the highest activity. These results demonstrate the selective role of GATA-4 in the transcriptional regulation of the IL-5 gene in a circumstance where multiple members of the GATA-binding proteins are expressed.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GATA2 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/GATA3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/GATA4 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0887-6924
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
11 Suppl 3
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
501-2
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9209438-Base Sequence,
pubmed-meshheading:9209438-Binding Sites,
pubmed-meshheading:9209438-Calcimycin,
pubmed-meshheading:9209438-Cell Line,
pubmed-meshheading:9209438-DNA-Binding Proteins,
pubmed-meshheading:9209438-GATA2 Transcription Factor,
pubmed-meshheading:9209438-GATA3 Transcription Factor,
pubmed-meshheading:9209438-GATA4 Transcription Factor,
pubmed-meshheading:9209438-Humans,
pubmed-meshheading:9209438-Interleukin-5,
pubmed-meshheading:9209438-Promoter Regions, Genetic,
pubmed-meshheading:9209438-Recombinant Proteins,
pubmed-meshheading:9209438-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:9209438-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9209438-T-Lymphocytes,
pubmed-meshheading:9209438-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9209438-Trans-Activators,
pubmed-meshheading:9209438-Transcription, Genetic,
pubmed-meshheading:9209438-Transcription Factors,
pubmed-meshheading:9209438-Transfection,
pubmed-meshheading:9209438-Zinc Fingers
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| pubmed:year |
1997
|
| pubmed:articleTitle |
Of the GATA-binding proteins, only GATA-4 selectively regulates the human IL-5 gene promoter in IL-5 producing cells which express multiple GATA-binding proteins.
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| pubmed:publicationType |
Journal Article
|