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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-7-29
|
| pubmed:abstractText |
Tyrosine kinase growth factor receptors and Ras/Raf/MEK/MAPK signalling have been implicated in the suppression as well as augmentation of programmed cell death. In addition, a Ras-independent role for Raf as a suppressor of programmed cell death has been suggested by the recent finding that Craf1 interacts with members of the Bcl-2 family at mitochondrial membranes. However, genetic studies of C. elegans and Drosophila, as well as the targeted mutagenesis of the murine Araf gene, have failed to support such a role. Here we show that mice with a targeted disruption in the Braf gene die of vascular defects during mid-gestation. Braf -/- embryos, unlike Araf -/- or Craf1 -/- embryos (L.W. et al., unpublished), show an increased number of endothelial precursor cells, dramatically enlarged blood vessels and apoptotic death of differentiated endothelial cells. These results establish Braf as a critical signalling factor in the formation of the vascular system and provide the first genetic evidence for an essential role of Raf gene in the regulation of programmed cell death.
|
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1061-4036
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
293-7
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:9207797-Animals,
pubmed-meshheading:9207797-Apoptosis,
pubmed-meshheading:9207797-Blotting, Northern,
pubmed-meshheading:9207797-Blotting, Southern,
pubmed-meshheading:9207797-Cell Differentiation,
pubmed-meshheading:9207797-Embryo, Mammalian,
pubmed-meshheading:9207797-Endothelium, Vascular,
pubmed-meshheading:9207797-Gene Expression Regulation, Developmental,
pubmed-meshheading:9207797-Gene Targeting,
pubmed-meshheading:9207797-Genotype,
pubmed-meshheading:9207797-Heterozygote,
pubmed-meshheading:9207797-Histocytochemistry,
pubmed-meshheading:9207797-Homozygote,
pubmed-meshheading:9207797-In Situ Hybridization,
pubmed-meshheading:9207797-Mice,
pubmed-meshheading:9207797-Mice, Transgenic,
pubmed-meshheading:9207797-Protein-Serine-Threonine Kinases,
pubmed-meshheading:9207797-Proto-Oncogene Proteins,
pubmed-meshheading:9207797-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:9207797-Signal Transduction,
pubmed-meshheading:9207797-Stem Cells
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Endothelial apoptosis in Braf-deficient mice.
|
| pubmed:affiliation |
Section on Genetics, National Institute of Mental Health/National Human Genome Research Institute, Bethesda, Maryland 20892, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|