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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1997-7-24
|
| pubmed:abstractText |
Using baculoviral and bacterial systems, we expressed biologically active recombinant rat IL-13 and generated neutralizing rat IL-13 antiserum. Recombinant rat IL-13 produced by baculovirus-infected insect cells stimulated proliferation of TF-1 premyeloid cell line and induced expression of 15-lipoxygenase mRNA in human peripheral blood monocytes. Antiserum generated by immunizing a rabbit with recombinant bacterial rat IL-13 specifically inhibited TF-1 proliferation induced by baculoviral rat IL-13 but did not neutralize human IL-13 mitogenic activity. Western blotting with anti-rat IL-13 serum revealed a approximately 12 kD protein band in supernatants of insect cells infected with recombinant baculovirus carrying the rat IL-13 cDNA. The availability of recombinant rat IL-13 and rat IL-13 antibodies should facilitate studying the role of IL-13 in rat models of human inflammatory disorders.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
27
|
| pubmed:volume |
235
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
529-32
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9207190-Animals,
pubmed-meshheading:9207190-Antibodies,
pubmed-meshheading:9207190-Baculoviridae,
pubmed-meshheading:9207190-Blotting, Western,
pubmed-meshheading:9207190-Cell Division,
pubmed-meshheading:9207190-Cell Line,
pubmed-meshheading:9207190-Cells, Cultured,
pubmed-meshheading:9207190-Humans,
pubmed-meshheading:9207190-Inflammation,
pubmed-meshheading:9207190-Interleukin-13,
pubmed-meshheading:9207190-Lymphocyte Activation,
pubmed-meshheading:9207190-Lymphocytes,
pubmed-meshheading:9207190-Molecular Weight,
pubmed-meshheading:9207190-Neutralization Tests,
pubmed-meshheading:9207190-Rabbits,
pubmed-meshheading:9207190-Rats,
pubmed-meshheading:9207190-Recombinant Proteins,
pubmed-meshheading:9207190-Spodoptera,
pubmed-meshheading:9207190-Transfection
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Expression of recombinant rat interleukin-13 (IL-13) and generation of a neutralizing rat IL-13 antiserum.
|
| pubmed:affiliation |
Renal Division, Emory University School of Medicine and Veterans Affairs Medical Center, Atlanta, Georgia 30033, USA. fglakki@emory.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|