Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
1997-8-5
pubmed:abstractText
Myotonic dystrophy (DM) is caused by the expansion of a trinucleotide repeat, CTG, in the 3' untranslated region of a protein kinase gene, DMPK. We set out to determine what effect this expanded repeat has on RNA processing. The subcellular fractionation of RNA and the separate analysis of DMPK transcripts from each allele reveals that transcripts from expanded DMPK alleles are retained within the nucleus and are absent from the cytoplasm of DM cell lines. The nuclear retention of DMPK transcripts occurs above a critical threshold between 80 and 400 CTGs. Further analysis of the nuclear RNA reveals an apparent reduction in the proportion of expansion-derived DMPK transcripts after poly(A)+ selection. Quantitative analysis of RNA also indicates that although the level of cytoplasmic DMPK transcript is altered in DM patients, the levels of transcripts from 59 and DMAHP, two genes that immediately flank DMPK, are unaffected in DM cell lines.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-1302022, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-1310900, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-1546325, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-1546326, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-1878973, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7477378, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7477379, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7543316, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7590731, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7691346, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7698644, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7758107, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7777532, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7874160, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7896884, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-7905855, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8036515, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8162011, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8288237, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8358430, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8469976, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8595416, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8596908, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8596916, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8612237, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8664297, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8673131, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8696339, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8789448, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8855655, http://linkedlifedata.com/resource/pubmed/commentcorrection/9207102-8875246
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7394-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Transcriptional abnormality in myotonic dystrophy affects DMPK but not neighboring genes.
pubmed:affiliation
Department of Genetics, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't